相关实验视频
Updated: Jul 12, 2025

08:17
A Proximal Culture Method to Study Paracrine Signaling Between Cells
Published on: August 28, 2018
9.5K
由子宫内膜细胞衍生出来的外体细胞通过IL-6 / JAK2 / STAT3通路促进腺菌症的发展
1School of Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China.
Experimental and therapeutic medicine
|October 23, 2023
概括
子宫内膜外体通过激活子宫内膜细胞中的Interleukin-6 (IL-6) 信号来促进腺化. 用tocilizumab阻断IL-6可以逆转这些影响,这表明IL-6向治疗腺菌症.
科学领域:
- 生殖生物学 生殖生物学
- 细胞生物学 细胞生物学
- 妇科疾病的发病因子
背景情况:
- 介质素-6 (IL-6) 在腺神病的发病过程中起作用.
- 精确的机制,特别是通过外体的细胞间通信,需要进一步研究.
研究的目的:
- 调查子宫内膜细胞衍生的外体是否通过IL-6信号在腺菌症中介于子宫内膜和子宫内膜之间的交叉声.
- 探索针对IL-6信号的治疗潜力.
主要方法:
- 从患有腺症的患者中分离出原发性腺性子宫肌细胞 (AM) 和eutopic子宫内膜细胞.
- 来自子宫内膜细胞的外体与AM细胞进行化,有或没有托西利祖马布 (IL-6抑制剂).
- 评估了细胞增殖,细胞亡,细胞周期,迁移以及IL-6/Janus激酶2 (JAK2) /STAT3通路蛋白质表达.
主要成果:
- 子宫内膜外抑制了AM细胞的亡,增加了增殖,S相过渡和迁移.
- 这些效应与增加的IL-6产生和JAK2/STAT3酸化有关.
- 托西利祖马布治疗完全逆转了这些外体诱导的变化,减少了IL-6和JAK2/STAT3信号传递.
结论:
- 由子宫内膜细胞衍生的外体通过IL-6/JAK2/STAT3激活促进AM细胞的增殖,迁移和细胞周期过渡.
- 这种外基因组介导的交叉介导促进了腺肌病的发展.
- 向IL-6代表了腺肌病的潜在补充治疗策略.
相关概念视频
The JAK-STAT Signaling Pathway
8.9K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
8.9K
Overview of Exosomes
2.7K
Exosomes are stable, lipid bilayer-enclosed vesicles capable of crossing biological barriers. They can carry a wide range of molecules required for intercellular communication. Once exosomes are released from the cell where they originated, they enter a recipient cell through various pathways such as fusion, receptor-mediated endocytosis, macropinocytosis, and phagocytosis.
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
Stahl et al. discovered exosomes in 1983, but the exosomes were initially considered waste products released from the...
2.7K
Oogenesis
63.8K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.8K
TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
Receptor Downregulation in MVBs
2.1K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.1K

