相关实验视频

Updated: Jul 12, 2025

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

Three-dimensional Confocal Analysis of Microglia/macrophage Markers of Polarization in Experimental Brain Injury

Published on: September 4, 2013

循环RNA PTP4A2通过STAT3调节微质极化,以促进缺血性中风中的神经炎症

Xingzhi Wang^1,2,3, Shenyang Zhang^1,2, Bingchen Lv^1,2

¹Department of Neurology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.

CNS neuroscience & therapeutics

|October 23, 2023

概括

循环RNA PTP4A2 (circPTP4A2) 通过通过STAT3.3.促进M1微质极化,驱动缺血性中风中的神经炎症. 击倒circPTP4A2可以减少大脑损伤,并促进有益的M2极化,提供潜在的治疗点.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

微质两极分化在神经炎症中至关重要,也是缺血性中风的潜在治疗点.
循环RNAs (circRNAs) 越来越多地被认为是它们在各种生物过程中的作用,包括疾病的发病.

研究的目的:

调查循环RNA PTP4A2 (circPTP4A2) 在缺血性中风后微质极化中的作用.
阐明涉及信号转换器和转录3 (STAT3) 激活器的潜在分子机制.

主要方法:

过渡性中脑动脉阻塞 (tMCAO) 在小鼠和氧气-葡萄糖剥夺/再输液 (OGD/R) 在微质细胞中被用于模拟缺血性中风.
circPTP4A2被使用shRNA晶状病毒击倒,STAT3酸化被调节.
评估了微质极化,炎症因素,心脏病发作量和脑血流量. RNA下拉和RIP测定证实了circPTP4A2和STAT3的结合.

主要成果:

在缺血性中风模型中,circPTP4A2水平升高.
通过circPTP4A2倒置,减少了心脏病发作量,改善了大脑血液流动,减轻了神经系统缺陷.
circPTP4A2的淘汰抑制了M1微质极化,促进了M2极化,并抑制了STAT3酸化,这表明了STAT3依赖的机制.

结论:

在缺血性脑损伤中,circPTP4A2通过促进STAT3依赖的微质极化来刺激神经炎症.
circPTP4A2 Knockdown减轻了脑缺血损伤,并增强了M2微质极化,将其确定为缺血性中风的潜在治疗标.

关键词:

在STAT3中,我们可以使用STAT3.环PTP4A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2C2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2C2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2A2 这是一种炎症炎症炎症炎症.缺血性中风中风微质细胞中的微质细胞

更多相关视频

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates

Published on: January 30, 2014

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Published on: July 23, 2012

相关概念视频

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a...