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卵细胞质量由低糖化FSH通过在小鼠毛囊发育期间增加细胞对细胞相互作用来增强

Aubrey Converse¹, Zhenghui Liu², Jai C Patel³

¹Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.

Development (Cambridge, England)

|October 23, 2023

概括

低糖化卵泡刺激激素 (FSH21) 增强了卵泡生长和卵细胞质量,远远超过完全糖化FSH24. 这表明,FSH糖形式丰度的转变有助于与年龄相关的生殖健康下降.

科学领域:

生殖生物学生殖生物学
内分泌学在内分泌学.
细胞生物学细胞生物学

背景情况:

毛囊刺激激素 (FSH) 存在于不同的甘油形式,这是由于其β子单元的N-糖化异质性.
低糖化FSH (FSH21) 在年轻女性中更为生物活性和丰富,而完全糖化FSH (FSH24) 的生物活性较低,并且随着年龄的增长而增加.
卵细胞质量随着年龄的下降,可能与FSH糖形式丰度的变化有关.

研究的目的:

调查FSH糖形 (FSH21和FSH24) 对卵泡生成和卵细胞质量的直接影响.
为了确定FSH糖形式丰度的转变是否有助于与年龄相关的卵细胞质量下降.

主要方法:

使用封装的实验室老鼠毛囊生长系统进行长期培养 (10-12天).
对FSH21和FSH24 (10 ng/ml) 对卵泡生长,雌激素分泌和卵细胞质量的影响进行了比较.
通过暂时抑制双向通信来评估FSH21介导的细胞间通信的作用.

主要成果:

与FSH24相比,FSH21显著增强了卵泡生长,雌激素分泌和卵细胞质量.
FSH21促进了在24小时内建立跨区域投射,差距连接和细胞间通信.
抑制FSH21介导的沟通消除了它对卵泡生长,雌激素分泌和卵细胞质量的积极影响.

结论:

在体外,FSH21通过增强早期的细胞间通信来促进卵泡生成和卵细胞质量.
随着生殖衰老,体内自然从FSH21转变为FSH24,可能会导致依赖于年龄的卵细胞质量下降.

关键词:

这是一种FSH糖形蛋白.鼠标鼠标是一个鼠标.蛋细胞质量质量卵巢的卵泡是卵巢的卵泡.跨区域预测跨区域预测

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