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Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution
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快捷路径条形码和早期聚合可扩展的多重单细胞减少表示CpG甲基化测序在单核酸分辨率

Liyao Mai1,2, Zebin Wen2, Yulong Zhang2

  • 1Department of Hepatobiliary Surgery II, Zhujiang Hospital, Southern Medical University, Guangzhou 510280, Guangdong Province, China.

Nucleic acids research
|October 23, 2023
PubMed
概括

研究人员开发了多重可扩展的单细胞减少表示双硫酸盐测序 (msRRBS) 以进行高效的DNA甲基化分析. 这种新方法提供了强大的,具有成本效益的单细胞甲基化概况,揭示了细胞异质性.

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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...