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Updated: Jul 12, 2025

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血液ChIP Xtra:一个扩展的数据库比较全基因组转录因子结合和基因表达特征在健康的人类干细胞/原始细胞子集和白血病细胞

Julie A I Thoms¹, Forrest C Koch², Alireza Raei²

¹School of Biomedical Sciences, University of New South Wales, Sydney, Australia.

Nucleic acids research

|October 23, 2023

概括

该BloodChIP Xtra数据库提供了全面的全基因组转录因子 (TF) 占用率和染色质数据,用于健康和急性髓性白血病 (AML) 血细胞. 本资源有助于理解血液形成和AML中的TF结合和基因调节.

科学领域:

血液学血液学血液学
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

了解转录因子 (TF) 结合和染色质可访问性对于造血和急性髓性白血病 (AML) 研究至关重要.
现有的数据库往往缺乏对罕见的原发性造血干细胞和祖细胞以及AML亚型的综合数据.
集成TF占用,染色质可访问性和基因表达数据对于下游应用是必不可少的.

研究的目的:

开发和介绍BloodChIP Xtra数据库,这是一个用户友好的资源,用于探索TF占用率和染色质数据.
在健康和AML细胞中提供关键的造血TFs和基因素标记的全基因组结合概况.
促进基因查询和监管网络的建设.

主要方法:

使用改进的方法,对TF占用率和染色质可访问性的全基因组分析.
来自原始细胞和AML细胞系的TF结合,染色质可访问性和基因表达的数据集成.
开发一个基于网络的数据库 (http://bloodchipXtra.vafaeelab.com/) 用于数据可视化和查询.

主要成果:

与其前身BloodChIP相比,BloodChIP Xtra包含了显著更多的数据集.
在健康和AML血液生成中增加了罕见初级细胞类型的数据可用性.
该数据库允许在健康和AML状态之间比较TF结合和染色质可访问性.

结论:

BloodChIP Xtra为研究血液形成和AML的研究人员提供了一个可访问的平台.
综合数据有助于构建TF基因和蛋白质-蛋白质网络.
本资源支持对血液疾病中特定细胞过程的基因关联的评估.

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