青春激素通过m6ARNA甲基化调节丝基因表达
Shuaiqi Liu1, Huan Tian1, Yusong Xu1
1College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Cellular and molecular life sciences : CMLS
|October 23, 2023
概括
青春激素模拟物 (JHA) 通过调节丝腺中的N6-甲基氨酸 (m6A) RNA修饰来影响Bombyx mori丝生产. 这揭示了昆虫发育中的激素作用的新机制.
科学领域:
- 分子生物学分子生物学
- 昆虫生理学 昆虫生理学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 青春激素 (JH) 对昆虫的发育和生理学至关重要.
- N6-甲基氨酸 (m6A) 是一个关键的RNA修饰调节基因表达.
- 昆虫中JH和m6A之间的相互作用在很大程度上是未被探索的.
研究的目的:
- 调查青春激素模拟物 (JHA) 和bombyx mori.中的m6A修饰之间的关系.
- 阐明m6A在调解JH对丝腺发育和子生产的影响中的作用.
主要方法:
- 在Bombyx mori幼虫中应用JHA.
- 转录组测序和m6后丝腺 (PSG) 的测序.
- 基因表达分析和验证通过METTL3敲击.
主要成果:
- JHA治疗延长了幼虫期,增加了子的重量/厚度.
- JHA显著改变了PSG中的m6A修饰模式,影响了与核酸结合和代谢相关的基因.
- 547个基因,包括16个与丝相关的基因 (例如,BmSPI4,BmSPI5),在m6A和表达水平上都受到JHA的调节.
结论:
- 在Bombyx mori后部丝腺中,对JH作用至关重要的一种修饰.
- JH通过m6对CDS区域的修饰直接调节与丝相关的基因 (BmSPI4,BmSPI5).
- 这项研究揭示了昆虫中由m6A介导的JH信号传递的新机制.
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