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核心基因组多部位序列类型化Clostridioides difficile以调查在医院环境中的传播

Paraskevas Filippidis^1,2, Laurence Senn¹, Fabrice Poncet^1,3

¹Infection Prevention and Control Unit, Infectious Diseases Service, Lausanne University Hospital and University of Lausanne, Lausanne, Switzerland.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

|October 23, 2023

概括

使用核心基因组多部位序列类型 (cgMLST) 的基因组类型改进了与医疗相关的Clostridioides difficile感染 (HA-CDI) 调查. 这种方法确定了无法解释的传播途径,增强了医院感染控制策略.

科学领域:

传染性疾病传染性疾病
基因组学就是基因组学.
流行病学流行病学

背景情况:

与医疗保健相关的Clostridioides difficile感染 (HA-CDI) 是一个重大挑战.
传统的HA-CDI流行病学方法往往不足以进行详细的传播追踪.

研究的目的:

评估一个分阶段的方法,结合二次隔离,使用核心基因组多部位序列类型 (cgMLST) 的单个毒性菌群的基因组类型和流行病学数据分析.
为了调查C. difficile在医院环境中的传播.

主要方法:

在6个月内对来自71名患者的毒性C. difficile阳性便样本进行了回顾性分析.
使用修改的双位置序列类型 (DLST) 进行初始类型化.
随后使用cgMLST进行基因组比较和聚类,然后对电子行政记录进行流行病学联系的分析.

主要成果:

发现了86个C. difficile分离体,其中13种不同的DLST类型在多名患者中共享.
通过cgMLST (具有≤3位点差异的隔离物) 确定了7个基因组群.
在7个基因组集群中,只有4个确定了可信的流行病学联系.

结论:

大约40%的cgMLST定义的集群有不明原因的HA-CDI收购,这表明未识别的传播途径,无症状的携带者或社区储库.
DLST和cgMLST的组合提供了一种有希望的,具有成本效益的策略,用于在医院调查CDI传播.

关键词:

细菌基因组学是一种细菌基因组学.克洛斯特里迪奥伊德斯困难菌流行病学流行病学分子类型的定型.疫情爆发调查调查.cgMLST 的意思

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