多中心跨实验室对常规系统的研究,用于测试特莫西林的敏感性,使用多药耐药菌株的挑战小组进行测试

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Corentin Deckers¹, Florian Bélik², Olivier Denis²

¹Laboratory of Clinical Microbiology, CHU UCL Namur, UCLouvain, Site Godinne, Avenue Gaston Therasse, 1, 5530, Yvoir, Belgium. corentin.deckers@uclouvain.be.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology

|October 23, 2023

概括

准确的泰莫西林 (TMO) 敏感性测试对于治疗多药耐药性肠杆菌感染至关重要. 大多数常规方法表现良好,但在Proteae物种中发现了差异.

科学领域:

临床微生物学临床微生物学
抗生素敏感性测试抗生素敏感性测试
传染性疾病传染性疾病

背景情况:

准确的泰莫西林 (TMO) 敏感性测试对于有效治疗多药耐药性肠道细菌引起的感染至关重要.
对临床实验室来说,评估常用的敏感性测试方法的性能至关重要.

研究的目的:

调查常规泰莫西林敏感性测试对具有挑战性的Enterobacterales菌株的性能和可重复性.
将各种自动化系统和磁盘扩散方法与黄金标准的微稀释方法进行比较.

主要方法:

一项涉及12个比利时实验室的多中心研究分析了47个临床Enterobacterales分离物.
使用VITEK® 2,BD PhoenixTM,ETEST®和磁盘扩散方法进行的测试,与汁微稀释 (BMD) 相比.
使用EUCAST 2023标准解释结果;使用参考菌株评估可重复性.

主要成果:

基于MIC的方法的绝佳基本协议 (91.5-100%),不包括Proteae物种.
观察到的最高类别的协议是ETEST® (97.5%),其次是VITEK® 2 (93.2%),磁盘扩散 (91.6%),以及BD PhoenixTM (88.5%).
BD PhoenixTM和磁盘扩散显示出更高的超调电阻率;VITEK® 2和ROSCO平板有更多非常大的差异.

结论:

常规的铁莫西林敏感性测试方法通常为肠杆菌提供准确和可重复的结果.
特别是在Proteae物种中发现了差异,需要仔细解释.
可复制性与参考菌株有所不同,这突显了适当的质量控制的重要性.

关键词:

抗微生物敏感性测试试验抗微生物敏感性测试欧卡斯特的断点进入细菌体中的细菌.泰莫西林是一种药物.

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