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Updated: Jul 12, 2025

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
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红色光蛋白是从绿色光蛋白中改造出来的

Hiromi Imamura1, Shiho Otsubo2, Mizuho Nishida1

  • 1Division of Systemic Life Science, Graduate School of Biostudies, Kyoto University, Kyoto 606-8501, Japan.

Proceedings of the National Academy of Sciences of the United States of America
|October 23, 2023
PubMed
概括

研究人员通过引入特定突变将绿色光蛋白 (GFP) 改造成红色光蛋白 (RFP). 这项工作澄清了红色光体形成的机制,并使新的光蛋白发展成为可能.

关键词:
在X射线晶体学.光蛋白质是一种光蛋白质.现场成像 现场成像 现场成像蛋白质工程工程 蛋白质工程

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Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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