在形成动物菌的真菌中 rhodopsin 循环酶的多样性
Matthias Broser1, Wayne Busse1, Anika Spreen1
1Institute of Biology, Department of Experimental Biophysics, Humboldt-Universität zu Berlin, Berlin 10115, Germany.
概括
研究人员在真菌中探索了Rhodopsin-guanylyl cyclases (RGCs),发现了红敏变体和近红外吸收neorhodopsins (NeoRs). 这揭示了真菌光受体的多样性和新型分子工具的潜力.
科学领域:
- 菌类学 菌类学是指菌类学.
- 摄影生物学 摄影生物学
- 分子生物学分子生物学
背景情况:
- 在真菌中,光感知是由罗多-瓜尼利环酶 (RGCs) 介导的.
- 在*Rhizoclosmatium globosum*中发现的异构体RGC包括neorhodopsin (NeoR) 和一种可视光吸收的Rhodopsin.
- 神经素 (NeoRs) 呈现出不寻常的近红外吸收和光.
研究的目的:
- 在早期分支的真菌中全面分析RGC基因的分布.
- 描述真菌RGC的光谱特性和进化起源.
- 探索真菌光受体在开发新分子工具方面的潜力.
主要方法:
- 对RGC基因分布的遗传数据的生物信息分析.
- 对本地和工程RGC变体的光谱分析.
- 突变研究和木乃伊构造来研究蛋白质功能.
主要成果:
- 识别了红敏的同位体RGCs (峰值激活~600nm) 用于GTP转化为cGMP的红光控制.
- 透露的异构RGCs通过基因重复进化在Chytridiales中,激活光谱从480-600nm.
- 已证明的新罗多素 (NeoRs) 吸收近红外 (641-721nm) 的光,扩大了已知的罗多素的光谱范围.
- 发现循环酶域对于同型和异型分子功能至关重要,可通过光传感器交换进行光谱调整.
结论:
- 的RGC表现出显著的光谱多样性,包括红移和近红外吸收变体.
- 循环酶域在二元化中的作用允许对光谱灵敏度进行模块化适应.
- 菌罗多普辛为创造具有从UVB到近红外光谱灵敏度的新型分子工具提供了广泛的潜力.
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