维生素D3介导的酸 - 酸自组装:制造,稳定和改善细胞运输
Zheng Fang1, Xiaoping Wu2, Fangfang Wang2
1Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.
Food chemistry
|October 23, 2023
概括
一种新的--维生素D3输送系统增强了细胞运输. 该系统保护维生素D3并改善的吸收,为营养物质提供提供双重效益的方法.
科学领域:
- 生物材料科学 生物材料科学
- 营养科学 营养科学
- 细胞生物学 细胞生物学
背景情况:
- 和维生素D3是重要的营养素,在骨健康和整体生理学中起着至关重要的作用.
- 需要有效的输送系统来克服营养吸收和稳定性的挑战.
- 基于的输送系统为有针对性和增强的营养物质生物可用性提供了潜力.
研究的目的:
- 开发和描述一个自我组装的--维生素D3三元输送系统 (CSPH-Ca-VD3).
- 在模拟胃肠道消化过程中调查系统的稳定性.
- 评估CSPH-Ca-VD3在促进细胞运输中的有效性.
主要方法:
- 制备和描述CSPH-Ca-VD3纳米复合体 (大小,结构).
- 纳米复杂物形成的热力学分析 (光谱).
- 在模拟的胃肠道条件下评估稳定性.
- 在Caco-2细胞单层中测量运输效率.
- 与运输相关的基因表达的分析 (TRPV6,calbindin D9k,PMCA1b).
主要成果:
- CSPH-Ca-VD3形成稳定的球形纳米复合体 (135.2 ± 10.2 nm),由疏水性相互作用驱动.
- 该系统表现出极好的稳定性,防止维生素D3的降解,增强的溶解性.
- 与自由Ca2+相比,Caco-2细胞之间的运输增加了2.3倍.
- 在用CSPH-Ca-VD3治疗的Caco-2细胞中观察到TRPV6,calbindin D9k和PMCA1b表达的升调.
结论:
- CSPH-Ca-VD3三元系统是提高生物可用性的有希望的方法.
- 该系统有效地保护维生素D3免受降解,并促进的吸收.
- 这项研究为开发先进的酸酸输送系统提供了基础.
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