定量蛋白质组学揭示了血蛋白质概况和肺结核患者的潜在途径,无论是患有糖尿病还是没有糖尿病
Xinxin He1, Yunguang Wang2, Yue Yang3
1Department of Nephrology, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Traditional Chinese Medicine), Hangzhou, Zhejiang, 310000, PR China; School of Clinical Medicine, Hangzhou Medical College, Hangzhou, Zhejiang, 311399, PR China.
Tuberculosis (Edinburgh, Scotland)
|October 23, 2023
概括
糖尿病 (DM) 影响肺结核 (PTB) 患者的蛋白质概况,影响免疫反应和代谢途径. 这项研究揭示了DM和PTB同时存在的患者的关键蛋白质差异.
科学领域:
- 蛋白质组学是指蛋白质组学.
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
背景情况:
- 肺结核 (PTB) 和糖尿病 (DM) 的综合负担对全球健康构成了重大挑战.
- 虽然DM增加了PTB风险,而PTB可能会恶化DM的进展,但分子相互作用仍然不清楚.
研究的目的:
- 为了研究DM对PTB患者的血蛋白质组的影响.
- 识别具有和没有DM的PTB中差异表达蛋白 (DEPs) 和相关途径.
主要方法:
- 利用基于数据独立获取 (DIA) 的液体染色学-并联质谱法 (LC-MS/MS) 进行等离子体蛋白质组分析.
- 对比了PTB患者,DM与PTB患者结合的蛋白质概况,以及健康对照.
- 对已识别的DEP进行生物信息学分析.
主要成果:
- 已经确定了268个DEP.
- 与健康对照组相比,PTB和DM-PTB组的DEP主要与免疫反应,补充和凝血级联以及胆固醇代谢途径有关.
结论:
- 蛋白质组分析揭示了DM患者的PTB蛋白质特征和途径变化.
- 这些发现提供了对DM和PTB之间的相互作用背后的分子机制的见解.
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