普通鱼 (Cyprinus carpio) CD81通过调节自和RLRs-干扰素信号通路促进CyHV-3病毒的复制
Xuezhu Lee1, Zihan Fan1, Zhihong Huang1
1College of Marine Sciences of South China Agricultural University & Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, PR China.
Fish & shellfish immunology
|October 23, 2023
概括
常见的鱼CD81蛋白质通过调解自和抑制抗病毒反应,促进了科伊疹病毒 (KHV) 的复制. 减少CD81阻碍病毒传播,提供了对KHV感染机制的见解.
科学领域:
- 病毒学 病毒学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 类疹病毒3型 (CyHV-3),或科伊疹病毒 (KHV),导致类虫的死亡率和经济损失显著.
- 病毒,包括CyHV-3,必须克服宿主抗病毒防御以建立感染.
- 众所周知,诸如CD81之类的tetraspanins可以影响病毒感染过程.
研究的目的:
- 调查常见鱼CD81 (CcCD81) 在CyHV-3感染中的作用.
- 为了描述CcCD81基因和蛋白质结构.
- 阐明CcCD81影响CyHV-3复制和宿主抗病毒反应的分子机制.
主要方法:
- 克隆和表征CCCD81基因和蛋白质.
- 对CCCD81表达的组织分布分析.
- CcCD81 在常见鱼细胞 (CCB) 中进行了淘汰实验,随后感染了CyHV-3.
- 病毒基因表达的分析 (VP136).
- 评估自水平. 自水平的评估.
- 测量ISRE和IFN-1促进者的活动.
- 对关键的干扰素信号通路基因 (RIG-1,MDA5,MAVS,TBK1,IRF3) 的评估.
主要成果:
- CcCD81基因编码了一种234氨基酸蛋白质,具有四个跨膜域,在鱼组织中广泛表达,特别是在头部脏和大脑中.
- 在CyHV-3感染后的CCB细胞中,CCCD81的表达迅速增加.
- 击败CCCD81减少了病毒基因VP136的表达.
- CcCD81的敲除显著降低了自,并增强了ISRE和IFN-1的促进活性,以及RIG-1-介导的抗病毒信号通路基因.
- 发现CCCD81能够调节自并抑制RIG-1-介导的抗病毒信号传递,从而对I型干扰素的产生产生产生负面调节.
结论:
- CcCD81在促进CyHV-3复制方面发挥着至关重要的作用.
- 该蛋白质通过调解自和抑制依赖RIG-1的抗病毒免疫反应来促进病毒感染.
- 这些发现揭示了自,四氨酸和病毒病原体之间的新鲜联系,有助于理解CyHV-3感染机制.
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