乌斯蒂拉戈梅迪斯的一种转录激活因子,在致病原体诱导的瘤形成过程中调节玉米的增生
Weiliang Zuo1, Jasper R L Depotter2,3, Sara Christina Stolze4
1Institute for Plant Sciences and Cluster of Excellence on Plant Sciences (CEPLAS), University of Cologne, Cologne, 50674, Germany. wzuo@uni-koeln.de.
Nature communications
|October 23, 2023
概括
乌斯蒂拉戈·梅迪斯真菌通过形成瘤引起玉米常见污垢. 它的效应蛋白Sts2激活了叶子发育基因,促进瘤细胞分裂和植物疾病.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 遗传学 遗传学 是一个
背景情况:
- 玉米常见污垢,由Ustilago maydis引起,涉及空中植物部位的瘤形成.
- 驱动这种瘤发生的分子机制尚未完全理解.
- 了解这些机制是开发抗病策略的关键.
研究的目的:
- 为了研究乌斯蒂拉戈可能的效应蛋白Sts2在玉米常见污垢发展中的作用.
- 为了阐明Sts2在植物病原体相互作用期间的分子功能.
- 确定与Sts2相互作用的植物因素及其在瘤发生中的作用.
主要方法:
- 关于U. maydis效应器Sts2.的表征.
- 对Sts2转移到玉米细胞的分析及其核功能.
- 确定Sts2的植物相互作用伙伴,例如ZmNECAP1.1.
- 叶子发育调节者的基因表达分析.
- 使用主导-负抑制 (SRDX-动机融合) 的功能分析.
主要成果:
- Sts2被转移到玉米核中,并充当转录激活剂,对毒性至关重要.
- Sts2与植物转录激活剂ZmNECAP1.1相互作用.
- Sts2激活了叶子发育调节者的表达,促进了瘤的形成.
- 主导负抑制Sts2功能可以防止瘤的发展,证实了它的关键作用.
结论:
- Sts2是Ustilago maydis的关键毒性作用因子,通过操纵宿主基因表达来驱动玉米的常见污染.
- Sts2和ZmNECAP1之间的相互作用,以及随后的叶子发育调节者的激活,对于病原体诱导的瘤形成至关重要.
- 这项研究揭示了植物瘤发生的分子基础和真菌病原体中的效应器功能的新见解.
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