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在胡羊的产后骨肌肉生长期间,全基因组的表观遗传动力学

Yutao Cao^1,2,3, Yue Ai^1,2,3, Xiaosheng Zhang⁴

¹Beijing Key Laboratory of Animal Genetic Improvement, College of Animal Science and Technology, China Agricultural University, Beijing, China.

Communications biology

|October 23, 2023

概括

表观遗传修饰,包括DNA甲基化和染色质可访问性,调节骨肌肉生长和纤维类型的变化在年轻的胡羊. 这项研究揭示了在产后发育期间参与肌肉缩和纤维转化的关键基因.

科学领域:

动物科学动物科学
遗传学是一个遗传学.
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

产后的骨肌肉发育涉及过度缩和纤维转化.
在这些过程中表观遗传修饰的作用仍然在很大程度上未被探索.

研究的目的:

调查胡羊在产后骨肌肉发育过程中的表观遗传动力学和基因表达变化.
确定关键的表观遗传调节剂和与肌肉生长和纤维类型转换相关的转录因子.

主要方法:

利用ATAC-seq,全基因组双硫酸盐测序和RNA-seq分析不同出生后年龄 (3天,3个月,6个月,12个月) 的胡羊的肌肉组织.
进行了差异基因表达分析,确定了差异可访问和甲基化区域,并进行了转录因子网络分析.

主要成果:

鉴定了6865个不同的表达基因,3742个不同的可访问区域和11799个不同的甲基化区域.
与肌肉发育途径相关的特异性表观遗传变化,特别是在3天至6个月之间.
突出转录因子ARID5B,MYOG和ENO1用于肌肉缩,NR1D1,FADS1,ZFP36L2和SLC25A1用于纤维转化.

结论:

DNA甲基化和染色质可访问性在调节产后骨肌肉生长和胡羊纤维转化方面发挥着至关重要的作用.
表观遗传机制有助于肌肉缩和纤维类型变化的独特发育轨迹.

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