相关实验视频
Updated: Jul 12, 2025
11:53
Substrate Generation for Endonucleases of CRISPR/Cas Systems
Published on: September 8, 2012
27.4K
Cas13对宿主RNA的内在向限制了它的实用性
Zexu Li1,2,3, Zihan Li1,2,3, Xiaolong Cheng4,5
1Key Laboratory of Bioresource Research and Development of Liaoning Province, College of Life and Health Sciences, Northeastern University, Shenyang, China.
Nature biomedical engineering
|October 23, 2023
概括
Cas13酶可以通过新的机制意外地准哺乳动物细胞中的宿主RNA,独立于引导RNA序列. 这种内在的RNA向影响着lentiviral传递和表达,需要对Cas13应用进行仔细考虑.
科学领域:
- 分子生物学分子生物学
- 基因编辑 基因编辑
- 在RNA生物学,RNA生物学.
背景情况:
- Cas13酶是RNA引导的核酶,在RNA淘汰,编辑,成像和治疗中具有应用.
- 以前对Cas13功能的理解主要集中在其由CRISPRRNA (crRNA) 指导的cis/transRNA分裂活动上.
研究的目的:
- 调查Cas13在哺乳动物细胞中对宿主RNA的内在向之前未被理解的机制.
- 描述Cas13介导宿主RNA裂变的转录特异性和依赖于构造的性质.
- 评估内在宿主RNA向对lentiviral输送和Cas13系统的表达的影响.
主要方法:
- RNA免疫沉测序 (RIP-seq) 是一种测序方法.
- 转录组的概况分析
- 生物化学分析 生物化学分析
- 高通量选的高通量选
- 机器学习 机器学习
- 隐形病毒系统 (单向量和双向量)
主要成果:
- 在哺乳动物细胞中,Cas13表现出宿主RNA的内在向,通过与标准crRNA引导裂变不同的机制.
- 宿主RNA被Cas13分裂是转录特异的,crRNA独立的,并且动态依赖于Cas13效应物的构造状态.
- 参与病毒过程的宿主基因,当被Cas13准时,会限制lentiviral传递和Cas13系统的表达.
- 在单向量和双向量晶状病毒系统中分析了几种Cas13家族效应因子.
结论:
- 在哺乳动物细胞中,Cas13具有未知的内在RNA向能力.
- 这些发现需要谨慎对待基于Cas13的应用程序中的意外宿主RNA向,特别是使用lentiviral系统的应用程序.
- 为适当使用晶状病毒Cas13系统提供了指导,强调需要考虑内在向效应.
更多相关视频
10:16Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
Published on: August 16, 2024
1.2K
07:59Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
Published on: April 25, 2025
311
相关概念视频
02:53CRISPR and crRNAs
17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
01:23RNA Interference
26.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.1K
02:30siRNA - Small Interfering RNAs
16.8K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.8K
02:15Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
01:23The Antiviral System of Bacteria and Archaea: CRISPR
31
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
31
01:59CRISPR
52.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.0K