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相关概念视频

Ligand Binding and Linkage00:49

Ligand Binding and Linkage

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Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
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Ligand Binding Sites02:40

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Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
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Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

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Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
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Updated: Jul 12, 2025

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
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一个基于PYR1的正交CID模块,具有可重编程的联结特异性.

Sang-Youl Park1,2, Jingde Qiu1,2, Shuang Wei3

  • 1Department of Botany and Plant Sciences, University of California, Riverside, Riverside, CA, USA.

Nature chemical biology
|October 23, 2023
PubMed
概括

科学家们设计了新的化学诱导二分化 (CID) 模块来重新编程植物激素 (酸) 受体. 这项创新使得使用活生物传感器和合成生物学应用,能够灵敏地检测污染物.

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科学领域:

  • 合成生物学 合成生物学
  • 分子生物学分子生物学
  • 植物科学 植物科学

背景情况:

  • 植物利用酸 (ABA) 信号通路,涉及像PYR1这样的受体和像HAB1.1这样的酸酶.
  • PYR1受体系统允许重编程连接体识别,提供独特的合成生物学潜力.
  • 现有的系统缺乏对角性和多通道功能,无法应用于复杂的传感应用.

研究的目的:

  • 设计和验证一个直角化学诱导二分化 (CID) 模块,以扩展PYR1受体系统.
  • 创建基于PYR1的新型生物传感器,对特定配体具有高灵敏度.
  • 展示这些生物传感器的应用,用于检测被禁止的有机酸盐污染物和构建遗传电路.

主要方法:

  • 一个直角的"*"CID模块的设计,具有二度接口盐桥.
  • 进行X射线结晶学,生物化学测定和体内分析以确认模块正交.
  • 在Arabidopsis thaliana和Saccharomyces cerevisiae中构建和测试PYR1* MANDI/HAB1*和PYR1* AZIN/HAB1*系统.

主要成果:

  • 正交"*"模块已成功设计和验证.
  • 工程PYR1*MANDI/HAB1*和PYR1*AZIN/HAB1*系统表现出纳米分子联体敏感性.
  • 已经证明了对有机酸盐污染物的敏感检测,以及在生物体中构建多输入/输出遗传电路.

结论:

  • 新型联体可编程CID模块显著扩大了合成生物学工具的功能.
  • 这些进步使得开发新的基于植物和微生物的传感方式成为可能.
  • 开发的系统为多道化学传感和遗传电路构建提供了一个多功能平台.