在炎症风暴后,法维皮拉维尔可以保护肠细胞免受细胞死亡
Ugur Ozgurbuz1, Hilal Kabadayi Ensarioglu2, Damla Akogullari Celik2
1Anesthesiology and Reanimation, Izmir Ataturk Research and Training Hospital, İzmir, TUR.
Cureus
|October 24, 2023
概括
费维皮拉维尔可能会影响肠道肠道细胞中细胞死亡途径. 在炎症发生之前给予法维皮拉维尔显示出保护作用,而炎症后的治疗可能会增加亡.
科学领域:
- 胃肠病学 胃肠病学
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- 炎症性肠病 (IBD) 治疗越来越有针对性,强调早期免疫修饰疗法.
- 在IBD治疗期间,控制病毒感染至关重要.
- 在炎症期间,法维皮拉维尔对肠细胞的影响尚不清楚.
研究的目的:
- 为了研究法维皮拉维尔在诱导炎症后对肠肠细胞 (IEC-6细胞) 的影响.
- 分析法维皮拉维尔在炎症肠细胞中亡和亡途径中的作用.
主要方法:
- IEC-6细胞暴露在瘤亡因子-α (TNF-α) 和干扰素-γ (IFN-γ) 的作用下,以创建炎症模型.
- 细胞在炎症诱导前 (预防性) 或在炎症诱导后 (治疗) 用法维皮拉维尔治疗.
- 试验包括细胞毒性的MTT,蛋白质定位的间接免疫过氧化酶染色 (caspase 1,caspase 3,IL6,IL8,MLKL,RIPK1,TNF-α) 和细胞因子水平的ELISA.
主要成果:
- 发炎后的法维皮拉维尔治疗增加了卡斯巴酶1和卡斯巴酶3的活性,这表明它在亡中发挥了作用.
- 预防性法维皮拉维尔似乎调节了炎症性卡斯帕斯路径.
- 随着法维皮拉维尔的使用,中间蛋白-8 (IL8) 水平显著增加.
- 死亡亡标志物 (MLKL,RIPK1) 在接受法维皮拉维尔治疗的组中升高,特别是在炎症发作后.
- 与炎症模型相比,法维皮拉维尔治疗降低了TNF-α水平.
结论:
- 在炎症发生之前服用法维皮拉维尔可以通过调节炎性卡斯帕斯路径来保护肠道肠细胞.
- 在炎症后,法维皮拉维尔可能会诱导细胞死亡,主要是通过亡而不是肠细胞中的亡.
- 费维皮拉维尔可能会触发IL6释放并增强IL8相互作用,影响炎症途径.
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