相关实验视频

Updated: Jul 12, 2025

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
09:25

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

Published on: January 9, 2015

46.2K

在CBC-ALYREF复合体的冷-EM结构

Bradley P Clarke, Alexia E Angelos, Menghan Mei

    bioRxiv : the preprint server for biology
    |October 24, 2023
    PubMed
    概括

    核帽结合复合体 (CBC) 招募mRNA出口因子,如ALYREF,以促进基因表达. 这项研究揭示了这种相互作用的结构基础,澄清了mRNA处理和出口机制.

    更多相关视频

    High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE13:28

    High-resolution Single Particle Analysis from Electron Cryo-microscopy Images Using SPHIRE

    Published on: May 16, 2017

    50.4K
    Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction12:38

    Structure of HIV-1 Capsid Assemblies by Cryo-electron Microscopy and Iterative Helical Real-space Reconstruction

    Published on: August 9, 2011

    17.5K

    相关概念视频

    Cryo-electron Microscopy01:28

    Cryo-electron Microscopy

    Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
    3.3K