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Updated: Jul 12, 2025

Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction
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Do's and Don'ts of Cryo-electron Microscopy: A Primer on Sample Preparation and High Quality Data Collection for Macromolecular 3D Reconstruction

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在CBC-ALYREF复合体的冷-EM结构.

Bradley P Clarke, Alexia E Angelos, Menghan Mei

    bioRxiv : the preprint server for biology
    |October 24, 2023
    PubMed
    概括

    核帽结合复合体 (CBC) 招募mRNA出口因子,如ALYREF,以促进基因表达. 这项研究揭示了这种相互作用的结构基础,澄清了mRNA处理和出口机制.

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    相关概念视频

    Cryo-electron Microscopy01:28

    Cryo-electron Microscopy

    Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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