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不对称的皮层投射到条形直接和间接的途径明显控制行动
Jason R Klug1,2, Xunyi Yan1,3,2, Hilary A Hoffman1
1Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
bioRxiv : the preprint server for biology
|October 24, 2023
概括
研究人员绘制了控制运动的大脑电路. 他们发现,大多数皮质输入到D2-脊状投射神经元 (SPNs) 也连接到D1-SPNs,揭示了这些通路在行为中的不同角色.
科学领域:
- 神经科学是一个神经科学.
- 系统神经科学 系统神经科学
- 发动机控制器的控制器
背景情况:
- 基底,特别是条形体,对于通过直接和间接途径进行作用控制至关重要.
- 了解皮层输入到条状D1和D2脊状投射神经元 (SPNs) 的收和分歧对于阐明基底腺功能至关重要.
- 在调节行为方面,特定途径的皮质前皮层投影的确切作用在很大程度上仍未确定.
研究的目的:
- 调查皮质输入的重叠和分离,以准条状D1-SPNs和D2-SPNs.
- 为了确定光遗传学刺激特定路径的皮质前皮质子电路如何影响不同的行为.
- 阐明非对称的皮质三角管连接对基底腺节电路和相关疾病的功能影响.
主要方法:
- 在小鼠中利用了一种新的G删除狂犬病系统来追踪单突触输入.
- 采用光遗传刺激技术,选择性地激活D1-SPN和D2-SPN投射皮质神经元.
- 评估了刺激这些特定的皮质干路径对运动,强化学习和序列行为的行为影响.
主要成果:
- 发现超过三分之二的激发性输入到D2-SPNs也投射到D1-SPNs,表明了重要的输入重叠.
- 相反,只有大约三分之一的D1-SPN输入也针对D2-SPN,证明了不对称的连接性.
- D1-SPN-和D2-SPN-投射皮质神经元的光遗传激活差异调节了运动,强化学习和序列任务,突出了功能分歧.
结论:
- 皮层投射到条纹D1-和D2-SPNs是部分分离的,但表现出不对称的重叠.
- 特定于路径的皮质前皮质子电路对不同的行为领域施加不同的控制.
- 这些发现对理解涉及皮质 - 基底腺节电路的神经和精神疾病有重大意义.
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