精确多重复合的新领域,由大规模并行单分子UltraPCRR实现

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Updated: Sep 29, 2026

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
11:09

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

Published on: October 23, 2011

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Janice H Lai, Jung Won Keum, Haeun G Lee

    bioRxiv : the preprint server for biology
    |October 24, 2023
    PubMed
    概括

    超PCR将反应分成数百万个隔离放大区,使得高度多重的核酸检测成为可能. 这种新的方法克服了传统的多重PCR中的竞争问题,为先进的基因组学分析铺平了道路.

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    相关概念视频

    Super-resolution Fluorescence Microscopy01:37

    Super-resolution Fluorescence Microscopy

    Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.