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Updated: Jul 12, 2025

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization
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Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

Published on: June 17, 2015

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在人体组织中通过RNAin situ杂交检测Mycobacterium tuberculosis

Kievershen Nargan, Threnesan Naidoo, Mpumelelo Msimang

    bioRxiv : the preprint server for biology
    |October 24, 2023
    PubMed
    概括

    RNAscope直接检测组织中的Mycobacterium结核病 (Mtb) mRNA,改善了超越Ziehl-Neelsen染色的结核病诊断. 这种方法在ZN阴性样本中揭示了Mtb mRNA,提供了新的诊断和研究途径.

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    Detection of Tissue-resident Bacteria in Bladder Biopsies by 16S rRNA Fluorescence In Situ Hybridization

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    Detection of Viral RNA by Fluorescence in situ Hybridization FISH10:16

    Detection of Viral RNA by Fluorescence in situ Hybridization FISH

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    相关概念视频

    In-situ Hybridization02:31

    In-situ Hybridization

    In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
    Types of probes and labels
    A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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    FISH - Fluorescent In-situ Hybridization02:07

    FISH - Fluorescent In-situ Hybridization

    Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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