工业法规规定了男性细菌细胞发展的早期阶段
Ayelen Moreno-Irusta1,2, Esteban M Dominguez1,2, Khursheed Iqbal1,2
1Institute for Reproductive and Developmental Sciences, University of Kansas Medical Center, Kansas City, KS.
bioRxiv : the preprint server for biology
|October 24, 2023
概括
TAF7L对男性生育能力至关重要,因为它在老鼠中缺少会导致由于丸发育和精子生产中断而导致不孕. 这种TAF7L的破坏导致生殖细胞中介性停止.
科学领域:
- 生殖生物学 生殖生物学
- 遗传学 是一个遗传学.
- 发展生物学 发展生物学
背景情况:
- 男性生殖细胞的发育依赖于精确的基因网络调节.
- 关键的转录调节者是TATA盒结合蛋白相关因子 (TAFs).
- TAF7L位于X染色体上,在丸发育中起作用.
研究的目的:
- 研究TAF7L在老鼠丸发育中的生物功能.
- 建立和描述TAF7L功能丧失的老鼠模型.
主要方法:
- 通过CRISPR/Cas9基因组编辑,通过准外子3来创建TAF7L功能丧失的老鼠模型.
- 引入了一种110bp的删除,导致了移和过早停止密码.
- 突变的生殖系传播得到证实.
主要成果:
- TAF7L缺乏没有影响怀孕或产后存活率.
- TAF7L中断导致男性不孕,丸发育受损,精子生产失败.
- 突变的生殖细胞在生殖细胞阶段表现出介质性停止,有性体形成缺陷和介质性性染色体失活.
结论:
- 在大鼠中,TAF7L对于正常的雄性生殖细胞发育至关重要.
- 在老鼠中观察到的表型比以前研究的TAF7L无小鼠更严重.
- 在精子生成过程中,TAF7L对半变异和性染色体调节至关重要.
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