相关实验视频

Updated: Jul 12, 2025

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective

Published on: June 16, 2022

开发化学定义的介质,用于乳球菌乳酸菌亚种. 乳糖YF11可以消除超的压力影响

Xiaoli Zhang¹, Xiaodan Liu¹, Nan Zhang¹

¹Inner Mongolia Key Laboratory of Biomass-Energy Conversion, School of Life Science and Technology, Inner Mongolia University of Science and Technology, 7 Aerding Street, Baotou, 014010 People's Republic of China.

|October 24, 2023

概括

化学定义介质 (CDM) 对乳球菌乳糖菌进行了优化,降低了透压,增加了细胞体积. 这种方法增强了微生物生理学研究,使用定义的培养条件.

科学领域:

微生物学微生物学
生物技术是生物技术.
生物工艺工程生物工艺工程

背景情况:

复杂培养介质 (CCM) 在微生物研究中存在挑战,原因是未定义的组件.
化学定义介质 (CDM) 为研究微生物生理学和多组学提供了可控的替代方案.
优化CDM对于准确和可重复的实验结果至关重要.

研究的目的:

设计和优化新的化学定义介质 (CDM) 对于乳球菌乳糖亚种. 牛奶公司 YF11.11.
为了减少CDM中超位压力的影响.
为了提高细胞体积作为CDM性能指标.

主要方法:

在CCM和Lactococcus lactis的体内/体外氨基酸含量的测量总氨基酸.
基于氨基酸测量设计了新的丰富的CDM.
利用实验的统计设计和料批发发酵来优化CDM.
使用细胞体积和平均透压 (AOP) 评估CDM性能.

主要成果:

优化CDM (F-CDM) 显著降低了平均透压 (AOP),从610mOsm/kg·H2O降至360mOsm/kg·H2O.
细胞体积从初始CDM (I-CDM) 的0.142μm3增加到F-CDM的0.198μm3,接近CCM水平 (0.206μm3).
与最初的设计相比,优化的CDM方法表现出更好的性能.

结论:

开发的设计和改进CDM的战略,加上统计方法和料批量种植,是有效的.
这种方法为CDM在微生物研究中的更广泛应用提供了一个有希望的途径.
优化的CDM可以在微生物生理学和多组学研究中带来更可靠的结果.

关键词:

氨基酸是氨基酸的组成部分.细胞体积细胞体积细胞体积化学定义的介质是指化学定义的介质.在Fed-batch文化中.乳球球菌 (lactococcus lactis) 是一种乳球菌.这种压力是奥斯莫斯压力.

更多相关视频

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods

Novel Production Protocol for Small-scale Manufacture of Probiotic Fermented Foods

Published on: September 10, 2016

A high-throughput method to globally study the organelle morphology in S. cerevisiae

A high-throughput method to globally study the organelle morphology in S. cerevisiae

Published on: March 2, 2009

相关概念视频

Microbial Growth Media

Microbial Growth Media

Microbial growth media are essential tools in microbiology, providing the nutrients and conditions necessary to cultivate and study microorganisms. These media are categorized by their composition, consistency, and functional roles, enabling researchers to investigate microbial physiology, behavior, and interactions.Types and Consistencies of Growth MediaGrowth media can be solid, liquid, or semisolid. Solid media, often agar-based, allow visible colony growth for isolation and enumeration.

Factors Influencing Microbial Growth: Osmolarity

Factors Influencing Microbial Growth: Osmolarity

Osmolarity is the measure of solute concentration in a solution. It plays a critical role in determining water availability for organisms. Water moves across semipermeable membranes through osmosis, flowing from regions of lower solute concentration (more dilute) to regions of higher solute concentration (more concentrated).In high-solute environments, microbial cells lose water, leading to dehydration and inhibited growth. The extent to which water is available to microbes in such environments...