用于骨关节炎治疗的皮奥格利塔加载的软骨向纳米细胞 (Pio@C-HA-DOs)
Junyan Chen1,2, Wuyan Xu2, Tianming Dai3
1Guizhou Medical University, Guiyang, 550025, People's Republic of China.
International journal of nanomedicine
|October 24, 2023
概括
基于氨酸的纳米粒 (C-HA-DOs) 有效地将皮奥格利塔输送到软骨中,用于骨关节炎治疗. 这种有针对性的方法增强了药物的疗效,并延迟了体外和体内疾病的进展.
科学领域:
- 生物材料科学 生物材料科学
- 药物输送系统 药物输送系统
- 骨关节炎研究 骨关节炎研究
背景情况:
- 氨酸 (HA) 是骨关节炎 (OA) 治疗的关键生物材料.
- 皮奥格利塔是一种PPAR-γ激动剂,具有抑制OA的潜力,但在达到有效的局部药物度方面面临挑战.
- 目前的局限性需要在OA治疗中使用皮奥格利塔的新型输送系统.
研究的目的:
- 构建基于 hialuronic 酸的软骨向纳米细胞 (C-HA-DOs) 持续提供pioglitazone.
- 评估这些新型纳米细胞在治疗骨关节炎的体外和体内疗效.
- 调查C-HA-DOs的潜力,以改善皮奥格利塔在OA中的治疗作用.
主要方法:
- 使用HA,WYRGRL和十二胺合成了C-HA-DO,随后进行了表征 (FT-IR,NMR,泽塔潜力,TEM).
- 评估了药物负载,持续释放动力学,生物相容性和状细胞影响 (氧化应激,基因表达).
- 在体内研究包括软骨向可视化 (IVIS) 和OA大鼠模型的组织学分析.
主要成果:
- 合成的C-HA-DOs表现出最佳的粒子大小,表面电荷和稳定性,在生理和酸性pH下具有持续的pioglitazone释放配置.
- C-HA-DOs证明了有效的冠状细胞吸收,增强的生物相容性,以及对氧化应激的保护作用,包括ROS降低和线粒体潜能恢复.
- 在体内成像证实了软骨向,用Pio@C-HA-DOs治疗显著降低了软骨降解标志物,并延迟了OA的进展.
结论:
- C-HA-DOs促进了向药物输送到关节软骨,增强了pioglitazone在骨关节炎的治疗疗效.
- 开发的纳米细胞提供了一个有前途的战略,用于持续和有针对性的pioglitazone在OA治疗.
- 这种方法代表了通过改善药物输送来管理骨关节炎的重大进步.
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