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当LH被添加到卵巢刺激协议时,母亲的microRNA概况发生变化:试点研究

Fani Konstantinidou^1,2, Martina Placidi³, Giovanna Di Emidio³

¹Department of Psychological Health and Territorial Sciences, School of Medicine and Health Sciences, "G. d'Annunzio" University of Chieti-Pescara, 66100 Chieti, Italy.

|October 24, 2023

概括

这项试点研究探讨了在体外受精 (IVF) 卵巢刺激期间人类卵细胞中的microRNA (miRNA) 概况. 将黄素激素 (LH) 添加到卵泡刺激激素 (FSH) 改变了miRNA的表达,这表明LH会影响卵细胞的能力.

科学领域:

生殖生物学生殖生物学
分子遗传学分子遗传学
在体外受精 (IVF)

背景情况:

卵泡刺激激素 (FSH) 是IVF卵巢刺激的标准,但黄素化激素 (LH) 的使用是有争议的.
微RNAs (miRNAs) 是细胞功能的关键调节者,包括淋巴腺素的产生和卵泡的发育.

研究的目的:

调查卵巢刺激方案中的重组LH (rLH) 是否会改变人类卵细胞中的miRNA配置文件.
为了识别受试管婴儿期间LH联合使用影响的特定miRNA和途径.

主要方法:

试点研究比较了来自两个群体卵细胞中的miRNA配置文件:单独rFSH与rFSH加rLH.
使用预先设计的miRNA面板和实时PCR进行卵细胞分析.

主要成果:

与rFSH-only组相比,在rFSH加rLH组中观察到几个miRNAs的显著上调或下调.
功能和网络分析表明路径的差异化调制,包括与Tp53相关的生存机制,受LH存在的影响.
鉴定了以前涉及生殖过程的特定差异表达的miRNA (例如hsa-miR-484,hsa-miR-222).

结论:

在卵巢刺激期间同时服用LH似乎会调节人类卵细胞中的miRNA配置文件.
这些miRNA变化表明LH对卵细胞能力和潜在的IVF结果至关重要的途径产生影响.
对这些miRNA的进一步研究可以阐明LH的作用并改善试管婴儿临床成功.

关键词:

在FSH中,FSH是FSH.在这个过程中,IVF IVF.LH LH LH LH LH LH LH LH LH LH LH LH LH LH LH 在Tp53的Tp53 不孕症不孕不育不孕不育不孕不育这是一个微型RNA.卵子细胞卵子细胞卵巢刺激卵巢刺激

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