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人类TIMP1是一种增长因子,可以提高卵细胞发育能力

Yolanda Manríquez-Treviño¹, Blanca Sánchez-Ramírez², Juan Alberto Grado-Ahuir¹

¹Facultad de Zootecnia y Ecología, Universidad Autónoma de Chihuahua (UACH), Perif. Fco. R. Almada Km. 1, Chihuahua 31453, Chihuahua, Mexico.

Biotech (Basel (Switzerland))

|October 24, 2023

概括

在体外成熟 (IVM) 培养中添加人类组织金属蛋白酶-1抑制剂 (TIMP1) 提高了牛卵细胞发育能力. 50 ng/mL的TIMP1度产生了最多的胚芽细胞,表明胚胎发育潜力增强.

科学领域:

生殖生物学生殖生物学
细胞和分子生物学细胞和分子生物学
生物化学生物化学

背景情况:

卵细胞发育能力对于受精和胚胎发育至关重要,在卵泡发育过程中通过积卵细胞复合体 (COC) 通信获得.
人类组织金属蛋白酶-1抑制剂 (TIMP1) 参与细胞生长,分化和细胞亡,这表明它可能在卵细胞成熟中发挥作用.

研究的目的:

为了研究TIMP1作为生长因子对牛COCs体外成熟 (IVM) 的影响.
为了确定TIMP1补充剂是否可以提高卵细胞发育能力.

主要方法:

在体外培养牛的COC,TIMP1的度各不相同 (50,100,150 ng/mL).
评估了COC紧缩,卵细胞介质进展 (二代相速率),皮质颗粒分布,以及随后的胚胎发育.
主要组件分析 (PCA) 用于确定最佳条件.

主要成果:

所有TIMP1治疗都改善了COC紧缩 (p < 0.05).
150 ng/mL的TIMP1导致更多的第II阶段卵细胞和I型皮质颗粒分布 (p <0.05).
在50 ng/mL的TIMP1中,产生的胚胎细胞发育试验中最大的胚胎细胞发育率 (p < 0.05),PCA确定为最佳条件.

结论:

补充TIMP1对IVM期间的牛卵细胞发育能力产生积极影响.
用于增强发育能力的最佳度,由囊胚形成和特定的细胞模式表明,是50 ng/mL TIMP1.
作为IVM文化的增长因素,TIMP1的作用需要进一步研究,以改善辅助生殖技术.

关键词:

时间表1 时间表1 在体外成熟的成熟.卵子细胞的能力能力.卵细胞成熟的过程卵细胞质量卵细胞质量

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