天然产品生物合成和耐药性中的TetR和OmpR家族调节剂
Rachit S Patil1, Siddhant Sharma1, Aditya V Bhaskarwar1
1Department of Biology, Indian Institute of Science Education and Research, Tirupati, India.
Proteins
|October 24, 2023
概括
这项研究分析了参与细菌特种二次代谢物 (SSM) 生物合成的TetR和OmpR转录调节器 (TRs). 了解这些TR可以了解抗菌素耐药性 (AMR) 和新型应用.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 转录调节器 (TRs) 对细菌基因表达和在压力下生存至关重要.
- 细菌信号系统利用受体和输出领域来感知刺激并调节转录.
- 专门的二次代谢物 (SSM),包括抗生素,受到TRs的严重调节.
研究的目的:
- 对TetR和OmpR/PhoB转录调节器家族进行全面的审查和序列结构分析.
- 研究这些TRs在专门的二次代谢物 (SSM) 生物合成和耐药性的作用.
- 识别新型的动机,并了解这些TR的监管机制,以应用于潜在的应用.
主要方法:
- 对大量的TetR (158,389) 和OmpR/PhoB (77,437) 家族序列的生物信息分析.
- 序列和结构分析以识别已知的和新的功能动机.
- 对有关TetR和OmpR/PhoB家族在细菌信号传递和代谢中的现有文献的综述.
主要成果:
- 在TetR和OmpR/PhoB家族中发现了显著的序列分歧.
- 发现了新的序列图案,除了已知的图案之外,对于TR功能和结构完整性至关重要.
- 突出了这些TRs在SSM生物合成之外的各种细菌细胞过程中的无处不在性质.
结论:
- 了解TetR和OmpR/PhoB TRs的多种机制是解开SSM生物合成的关键.
- 这些TR为探索打击抗菌素耐药性 (AMR) 的机制提供了丰富的资源.
- 利用这些TR的监管作用可以导致重要的制药,农业和工业应用.
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