在大鼠中,IL-4通过HIF-1α/Bcl-2/BNIP3通路抑制自,减轻CIRI
Yijun Suo1, Lu Zhang2, Yuanhang Che3
1Department of Neurology, Affiliated Hospital of Jiujiang University, Jiujiang, Jiangxi Province, China.
Acta neurobiologiae experimentalis
|October 24, 2023
概括
干白素-4 (IL-4) 通过抑制自而减轻大脑缺血-再输损伤 (CIRI) 在老鼠中. 这种治疗效果是通过抑制低氧诱导因子1-alpha (HIF-1α) /BNIP3通路的介导.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 病理学 病理学 病理学
背景情况:
- 大脑缺血-再输损伤 (CIRI) 是一种具有重大神经后果的严重疾病.
- 自在CIRI中起着复杂的作用,其调制是潜在的治疗标.
- 介质蛋白-4 (IL-4) 已经显示出缓解炎症和亡过程的潜力.
研究的目的:
- 在大鼠模型中研究IL-4对CIRI的保护机制.
- 为了确定IL-4是否通过抑制自而发挥其作用.
- 阐明在IL-4对CIRI的作用中涉及的特定分子通路.
主要方法:
- 建立大脑缺血-再输液损伤的老鼠模型.
- 给不同实验组服用IL-4和一个催氧诱导因子1-α (HIF-1α) 抑制剂 (2-甲氧 Estradiol,2ME2).
- 西方涂抹分析用于量化关键自标志物 (LC3,p62) 和信号分子 (HIF-1α,BNIP3) 的蛋白质表达水平.
- 评估神经功能障碍,神经细胞亡,脑梗塞体积和自细胞计数.
主要成果:
- 与未经治疗的CIRI组相比,IL-4治疗显著降低了神经功能障碍,神经元亡,脑梗塞体积和自细胞数量.
- 给予IL-4导致LC3II/LC3I比率下降,HIF-1α和BNIP3的蛋白质表达减少,p62表达增加.
- 通过2ME2抑制HIF-1α消除了IL-4的保护作用,表明该途径的关键作用.
结论:
- 干白素-4 (IL-4) 有效地改善大脑缺血-再输液损伤 (CIRI) 在老鼠.
- 在CIRI期间,IL-4通过抑制HIF-1α/BNIP3信号通路来抑制过度的自.
- 用IL-4准HIF-1α/BNIP3通路代表了管理CIRI的有希望的治疗策略.
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