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在泰国使用经过验证的intI1原料从污水池中获取intI1基因丰富性
Valentine Okonkwo1, Fabien Cholet1, Umer Z Ijaz1
1Department of Infrastructure and Environment, James Watt School of Engineering, University of Glasgow , Glasgow, United Kingdom.
Applied and environmental microbiology
|October 24, 2023
概括
泰国的污水池有助于抗菌素耐药性 (AMR) 基因的传播. 标准化对1类整体基因 (intI1) 的量化对于评估AMR风险至关重要.
科学领域:
- 环境微生物学环境微生物学
- 公共卫生 公共卫生
背景情况:
- 抗菌素耐药性 (AMR) 是一个日益增长的全球健康威胁.
- 废水处理系统,包括化池,是AMR基因的储存库.
- 污水池在泰国AMR基因传播中的作用未得到充分研究.
研究的目的:
- 在泰国调查污水池作为1类整体和AMR基因的来源.
- 评估不同定量PCR (Q-PCR) 起始选择对intI1基因量化的影响.
- 建立标准化的方法来监测AMR基因丰富性在污水池环境中.
主要方法:
- 使用定量PCR (Q-PCR) 来测量1类整体基因 (intI1) 的丰度.
- 根据其性能和可靠性,评估了各种已发布的intl1原料套.
- 来自泰国的污水池样本被分析以评估intI1基因的流行率.
主要成果:
- 污水池被证实是1类综合体的重要环境来源.
- 选择intI1原料对量化结果和风险评估产生了重大影响.
- 采用F3-R3初始化套件,证明了对intI1量化最优的性能.
结论:
- 污水池在泰国AMR基因的环境传播中发挥着重要作用.
- 对intI1基因量化方法的标准化,特别是原料选择,对于准确的风险评估至关重要.
- 经过验证的F3-R3初始化套件被推用于在未来的研究中进行一致的intI1量化.
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