发现微蛋白编码小开放阅读框架的集成工作流

相关实验视频

Updated: Jul 12, 2025

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

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Kevin Cao1, Yasamin Hajy Heydary1, Gregory Tong1

  • 1Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA 92617, USA.

STAR protocols
|October 24, 2023
PubMed
概括

我们开发了一种全基因组协议,用于识别哺乳动物中转化小开放读取框架 (smORF) 编码的微蛋白. 这种方法使用核糖体分析 (Ribo-seq) 来发现小蛋白质,从而使未来的功能研究成为可能.

关键词:
生物信息学是一种生物信息学.细胞生物学 细胞生物学细胞培养 细胞培养基因表达 基因表达基因组学就是基因组学.分子生物学分子生物学在RNAseqqq中使用.序列分析 序列分析测序测序是指测序的时间.

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Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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