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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

3.5K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.5K
Leaky Scanning02:28

Leaky Scanning

5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K

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相关实验视频

Updated: Jul 12, 2025

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

3.6K

发现微蛋白编码小开放阅读框架的集成工作流.

Kevin Cao1, Yasamin Hajy Heydary1, Gregory Tong1

  • 1Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA 92617, USA.

STAR protocols
|October 24, 2023
PubMed
概括
此摘要是机器生成的。

我们开发了一种全基因组协议,用于识别哺乳动物中转化小开放读取框架 (smORF) 编码的微蛋白. 这种方法使用核糖体分析 (Ribo-seq) 来发现小蛋白质,从而使未来的功能研究成为可能.

关键词:
生物信息学是一种生物信息学.细胞生物学 细胞生物学细胞培养 细胞培养基因表达 基因表达基因组学就是基因组学.分子生物学分子生物学在RNAseqqq中使用.序列分析 序列分析测序测序是指测序的时间.

更多相关视频

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
06:58

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Published on: October 7, 2021

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相关实验视频

Last Updated: Jul 12, 2025

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
08:23

De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

Published on: February 18, 2022

3.6K
An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

3.4K
Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
06:58

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

Published on: October 7, 2021

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 蛋白质组学是指蛋白质组学.

背景情况:

  • 小开放式读取框架 (smORF) 编码微蛋白,这是一个新的功能生物分子类别.
  • 了解微蛋白的功能需要识别它们的转化smORFs.

研究的目的:

  • 提出一个全基因组的协议,用于识别哺乳动物系统中翻译的smORF.
  • 建立一个研究微蛋白的基础方法.

主要方法:

  • 产生核糖体分析 (Ribo-seq) 数据的数据.
  • 在晶体翻译的转录组组合创建一个ORF数据库.
  • 对Ribo-seq数据进行计算分析,以得分smORF翻译.

主要成果:

  • 一个全面的协议,用于识别整个哺乳动物基因组的翻译smORFs.
  • 能够发现由smORFs编码的新型微蛋白.

结论:

  • 提出的协议对于调查微蛋白功能的初步步骤至关重要.
  • 通过包括smORF编码的微蛋白来促进已知的蛋白质组的扩张.