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宿主-病原体相互作用: Enterobacter cloacae 对不同的宿主细胞类型具有不同的粘附和入侵能力

Elisabet Frutos-Grilo¹, Vanessa Kreling², Andreas Hensel²

¹Departament de Genètica i de Microbiologia, Universitat Autònoma de Barcelona, Bellaterra, Spain.

|October 24, 2023

概括

肠杆菌 (Enterobacter cloacae) 引起严重的感染,并强烈粘附于泌尿道和肺细胞. 这项研究是首次研究其与人体细胞系的体外相互作用,揭示了特定组织的粘附和入侵模式.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
细胞生物学细胞生物学

背景情况:

迫切需要新的抗生素来对抗多药耐药细菌.
菌 (Enterobacter cloacae) 是一种格拉姆阴性细菌,会导致严重的尿路和肺部感染 (UTI).
之前对E.cloacae在尿路感染中的致病性研究仅限于生物信息学.

研究的目的:

为了研究E.cloacae与人类细胞系的体外相互作用.
监测早期宿主-病原体相互作用,包括细菌粘附和入侵.
建立一个合适的体外模型来研究E.cloacae与宿主细胞的相互作用.

主要方法:

流量细胞计粘附试验.
经典的细胞计数试验.
胺入侵试验. 胺入侵试验.
聚焦激光扫描显微镜. 聚焦激光扫描显微镜.
对突变菌株 (fimH,fimA,csgA) 的研究.

主要成果:

E. cloacae对膀,和肺细胞具有强烈的粘附性,与临床尿路感染和肺部感染相关性相关.
对皮肤细胞的附着性最小 (A-431),与肠道细胞没有显著的相互作用 (Caco-2,HT-29).
E. cloacae被所有测试的宿主细胞内部化,在膀和细胞中入侵率最高.
fimH和fimA突变物取消了对T24膀细胞的粘附;csgA删除没有影响.

结论:

E.cloacae表现出不同的粘附和入侵模式,这取决于目标人体细胞类型.
这些模式与细菌在引起尿路感染和肺部感染方面的临床相关性相关.
T24膀细胞提供了一个合适的体外系统,用于评估E.cloacae的粘附和入侵.

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