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相关概念视频

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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相关实验视频

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Fluorescence detection methods for microfluidic droplet platforms
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控制基于纸张的分析设备中的光读数.

Anna Luongo1,2, Alexander Ritter von Stockert3, Frank D Scherag1,2

  • 1Laboratory for Chemistry & Physics of Interfaces, Department of Microsystems Engineering (IMTEK), Albert-Ludwigs-Universität Freiburg, Freiburg 79110, Germany.

ACS biomaterials science & engineering
|October 24, 2023
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概括

这项研究通过使用光检测和抗蛋白水凝涂层来增强基于纸张的诊断. 这种组合改善了光学读数,并使灵敏,低成本,一次性诊断设备成为可能.

关键词:
在 POC POC POC 中.光是一种光.基于纸张的诊断系统.散射散射是一种散射.表面的修改表面的修改在 μPAD 中,PAD 值为

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科学领域:

  • 材料科学 材料科学 材料科学
  • 分析化学 分析化学
  • 生物医学工程 生物医学工程

背景情况:

  • 纸是一种低成本,环保的材料,适合一次性诊断.
  • 目前快速测试中的色度测试提供了有限的定性结果和高的检测值.
  • 基于纸张的诊断可以从颜色测量之外的改进的读取方法中受益.

研究的目的:

  • 研究光作为纸质诊断设备的读数工具.
  • 通过分析影响光传输,散射和光的纸张特性来优化光学读取.
  • 开发一种抗蛋白涂层,以防止分析物吸附并增强灵敏度.

主要方法:

  • 根据纸张特征 (厚度,含水量,自光,成分) 评估光学读取特性 (传输,散射,光).
  • 在现场开发并将光化学交联的水凝涂层 (N,N-二甲基烯胺共聚物) 应用于纸纤维上.
  • 测试了光检测和水凝涂层的联合系统,用于敏感的基于纸张的分析,包括侧流装置 (LFD) 应用.

主要成果:

  • 基于纸张的光分析表明,与色度测试中使用的酸纤维素相比,光学读数优越.
  • 水凝涂层有效地减少了由于吸附到纸纤维上的分析分子损失.
  • 光检测和蛋白质排斥涂层的综合方法使得基于纸张的敏感诊断分析成为可能.

结论:

  • 光检测为纸质诊断提供了更高的性能.
  • 抗蛋白质的水凝涂层对于保持分析物的完整性和提高试验灵敏度至关重要.
  • 这一战略为开发更灵敏,更可靠的一次性诊断设备铺平了道路.