微环境调节药物输送纳米粒子用于治疗和预防典型的生物膜诱导的口腔疾病
Leyi Xiao1, Mengge Feng1, Chen Chen1
1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, 430079, P. R. China.
Advanced materials (Deerfield Beach, Fla.)
|October 24, 2023
概括
一种新型的纳米粒子,绿色氨酸 (ICG) - 拉巴胺,有效治疗和预防口腔生物膜疾病,如牙周炎和植入物感染. 它通过使用光动力学和光热效应调节微生物细胞环境来起作用.
科学领域:
- 口腔微生物学 口腔微生物学
- 纳米医学是一种纳米医学.
- 生物材料是一种生物材料.
背景情况:
- 口腔是各种微生物的息地;失调会导致牙周炎和植入物感染等疾病.
- 黄金葡萄球菌和口腔链球菌生物膜是这些口腔疾病的关键病原体.
- 目前的治疗方法在有效管理这些复杂的生物膜诱导疾病方面面临挑战.
研究的目的:
- 开发和评估一个脂质体封装的绿色氨酸 (ICG) 和拉巴胺纳米颗粒 (ICG-拉巴胺) 用于治疗和预防口腔生物膜疾病.
- 研究ICG-拉帕米辛调节微生物-细胞微环境的机制.
- 在动物模型中评估ICG-rapamycin在植入物相关感染和牙周炎的体内疗效.
主要方法:
- 体封装ICG-拉帕米辛纳米颗粒的设计.
- 通过近红外 (NIR) 激光照射下的光动力学和光热机制评估抗菌作用.
- 通过测量细菌运动性和ATP水平来评估生物膜形成抑制.
- 分析纳米粒子对细胞炎症,巨细胞极化 (M2),TGF-β表达和细菌细胞化的影响.
- 在植入物感染和牙周炎动物模型中的体内测试.
主要成果:
- 在NIR照射下,ICG-拉帕米辛通过增加活性氧物种 (ROS) 和温度来证明抗菌性质.
- 纳米粒子通过增强细菌运动性和ATP水平来抑制生物膜的形成.
- 微生物与细胞相互作用的调节包括促进M2巨细胞的两极分化,上调TGF-β的调节,并增强细菌的细胞分裂.
- 在体内研究证实了ICG-rapamycin在植入物相关感染和牙周炎模型中的治疗和预防作用.
结论:
- ICG-拉帕米是一种有前途的治疗策略,用于生物膜诱导的口腔疾病.
- 该纳米粒子通过调节微生物细胞微环境,有效治疗和预防口腔感染.
- 这种方法为未来在牙科和口腔健康领域的临床应用提供了潜力.
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