基因组和转录组洞察海洋生物膜中复杂的病毒-原核生物相互作用
Kun Zhou1,2,3, Tin Yan Wong4, Lexin Long1
1Department of Ocean Science, The Hong Kong University of Science and Technology, Hong Kong, China.
The ISME journal
|October 24, 2023
概括
海洋病毒积极与生物膜中的核细胞相互作用,影响微生物群落. 生物膜细菌拥有比海水中的更多的防御基因,这表明生物膜塑造了病毒动态和微生物进化.
科学领域:
- 海洋微生物学 海洋微生物学
- 病毒学 病毒学
- 生物膜生态学的生态学
背景情况:
- 海洋生物膜中存在由原核生物主导的复杂微生物群落.
- 在海洋生物膜中,病毒和核细胞之间的生态作用和相互作用尚不清楚.
研究的目的:
- 为了研究海洋生物膜中的病毒 - 益生菌相互作用.
- 描述涉及这些相互作用的病毒和 prokaryotic 基因.
- 为了比较生物膜中的微生物防御策略与开放的海水.
主要方法:
- 来自84个海洋生物膜的元基因组数据的分析.
- 识别病毒-原蛋白连接和基因含量 (辅助代谢基因,限制-修饰系统,抗病毒防御基因).
- 对新建立的生物膜进行转录组分析,并与海水样本进行比较.
主要成果:
- 鉴定了2446种病毒-原核细胞连接,主要是乌罗病毒菌体和蛋白质细菌,蓝藻细菌和细菌之间的细菌.
- 揭示了复杂的病毒 - 主机网络,病毒感染多个主机,反之亦然.
- 检测到许多辅助代谢基因,限制-修改系统,以及 prokaryotic 抗病毒防御系统,在生物膜中具有活跃的基因表达.
- 与海水 prokaryotes 相比,生物膜 prokaryotes 的抗病毒防御基因显著更丰富和更明显.
结论:
- 海洋生物膜是病毒和 prokaryotes 的港湾,积极互动.
- 这些相互作用驱动了生物膜内核细胞抗病毒防御机制的进化.
- 生物膜可能充当防御能力强大的原核生物的储存库,在更广的海洋中潜在地调节病毒感染力.
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