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从Thinopyrum中介染色体7J^s中鉴定了一种新的叶子抗基因,并将其侵入小麦中

Guotang Yang^1,2, Na Zhang³, Willem H P Boshoff⁴

¹State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

|October 24, 2023

概括

来自Thinopyrum intermedium的一种新的叶子抗基因已成功地引入小麦. 该基因位于染色体臂7J^sS上,为小麦育种计划提供强大的抗病能力.

科学领域:

植物育种和遗传学植物育种和遗传学
提高作物质量提高作物质量
遗传学和基因组学遗传学和基因组学

背景情况:

小麦易患各种真菌病,包括叶子生,茎生和粉等.
薄皮龙中间体具有对抗疾病有价值的遗传资源.
将外来基因引入小麦是提高作物弹性的一个关键策略.

研究的目的:

来自Thinopyrum intermedium的新型叶子抗基因入普通小麦 (Triticum aestivum) 基因组.
开发和描述携带小麦育种耐药性位点的转位线.
在异种染色体段上绘制叶子抗位的精确位置.

主要方法:

染色体工程被用来创建转位线WTT26.
对多种病原体种族进行了广泛的疾病评估 (Puccinia triticina,Puccinia graminis f. sp. 三树,Blumeria graminis f. sp. 在这里可以看到. 这就是"三位一体" (tritici).
细胞遗传学和分子标记分析,包括特定位点的放大片段测序,用于描述转位和绘制位点图.
用辐射产生较小的转位段.

主要成果:

转位线WTT26表现出对叶子生的近乎免疫力,对茎生的高耐药性 (Ug99),以及对粉状菌的成年植物耐药性.
细胞遗传学分析证实T4BS·7J^sS染色体转位携带抗性位置.
用74个特定标记器构建了染色体7J^sS的精细物理地图,定义了6个容器.
一个新型的叶子耐性位点被精确地映射到染色体7J^sS.S.上的0-69.29 Mb区域.

结论:

一种来自Thinopyrum intermedium的新型叶子抗位已成功识别并绘制在染色体臂7J^sS.上的地图.
开发的转位线和相关标记器为小麦抗病育种提供了有价值的工具.
这种外来位的侵入显著提高了普通小麦的抗病特征.

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