从昆虫的内源性病毒元素中解脱昆虫的病毒组.
Paula Rozo-Lopez1, William Brewer2, Simon Käfer3
1Department of Microbiology, University of Tennessee, Knoxville, TN, 37916, USA. plopez2@utk.edu.
BMC genomics
|October 24, 2023
概括
在使用RNA测序时,昆虫基因组中的内源性病毒元素 (EVE) 可以被误认为是新病毒. 这项研究强调了EVE作为准确发现昆虫病毒多样性的重大挑战.
科学领域:
- 病毒学 病毒学
- 基因组学就是基因组学.
- 昆虫学 昆虫学是一门学科.
背景情况:
- 昆虫拥有大量尚未发现的病毒生物多样性.
- 高通量RNA测序推进了昆虫病毒组研究.
- 昆虫基因组中的内源性病毒元素 (EVE) 复杂化了使用RNA测序的病毒发现.
研究的目的:
- 为了研究土豆虫的病毒组,Macrosiphum euphorbiae,一个重要的农业害虫和植物病原体载体.
- 在虫的基因组中识别和区分外源病毒与内源病毒元素 (EVE).
主要方法:
- 利用rRNA-depletedRNA测序来分析单个虫中的微生物群落.
- 采用PCR查来评估当地虫群体中特定遗传病毒的流行程度.
- 使用纳米孔测序与Illumina校正生成了M. euphorbiae的基因组组合草案,以检测转录活跃的EVE.
主要成果:
- 检测到的读数来自昆虫特异性病毒,寄生虫病毒,植物致病病毒和通过RNAseq.的菌体.
- 基因组组件显示,一些已识别的病毒序列实际上是虫基因组中存在的内源性病毒元素 (EVE).
- 证明EVE在RNA测序数据中对虫病毒的错误识别有所贡献.
结论:
- 内源性病毒元素 (EVE) 可以导致在昆虫RNA测序研究中错误识别病毒.
- 对于准确地描述昆虫病毒的多样性来说,EVE的存在是一个广泛的挑战.
- 建议在未来的昆虫病毒组研究中仔细考虑EVE.
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