长期储存条件对实验性牙微裂的发展的影响
Gustavo De-Deus1, Fabio Nakao Arashiro2, Erick Miranda Souza3
1Department of Endodontics, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil.
概括
长期干燥储存牙显著增加牙微裂. 在水或甲中储存牙可以在三年内防止新的微裂形成,这对牙科研究至关重要.
科学领域:
- 牙科材料科学 牙科材料科学
- 生物材料工程 生物材料工程
- 口腔生物学 口腔生物学
背景情况:
- 提取牙的适当存储对于临床前研究至关重要.
- 了解储存条件对牙结构的影响对于可靠的实验结果至关重要.
- 牙微裂可以损害牙的完整性,并影响研究结果.
研究的目的:
- 评估不同长期储存介质 (形式素,水,干) 和时间对实验性牙微裂的发展的影响.
- 确定在牙科研究中保存牙结构的最佳存储方法.
- 为牙样本保存提供数据驱动的建议.
主要方法:
- 60个提取的前被分为三组 (n=20) 并存储在甲,水或干燥条件下.
- 用微型计算机断层扫描扫描了牙,抽取后72小时,30天,6个月和3年.
- 进行了统计分析,以评估储存介质和时间对牙缺陷发生的影响.
主要成果:
- 干燥储存条件在所有时间点都显著增加了新的牙微裂的发生率.
- 在整个3年的研究期间,在存储在水中的牙中没有观察到新的缺陷.
- 甲组中只有一个牙在6个月后出现了新的缺陷,没有进一步的发展.
结论:
- 干燥储存对取牙的结构完整性有害,促进牙微裂的形成.
- 储水提供了一种可靠的方法,可以在3年内保存取出的牙,而不会引发新的微裂.
- 甲素的储存对于短期保存也似乎是安全的,观察到的缺陷的发展最小.
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