相关实验视频
Updated: Jul 12, 2025
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Development of Compendium for Esophageal Squamous Cell Carcinoma
Published on: April 12, 2024
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通过向FOXA3,HOXC10促进食道状细胞癌的进展,并表明生存结果不佳
Xiaoting He1, Huiyu Wang1, Runjie Wang1
1Oncology Department, The Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi People's Hospital, Wuxi Medical Center, Nanjing Medical University, Wuxi, Jiangsu, 214023, China.
Heliyon
|October 25, 2023
概括
在食道癌中,HomeoboxC10 (HOXC10) 被上调,通过通过FOXA3.3.激活MAPK通路来促进瘤生长. 沉默HOXC10抑制癌症的进展,表明其作为诊断标记物的潜力.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 癌症研究 癌症研究
背景情况:
- 食道癌,特别是状细胞癌 (ESCC),是全球癌症相关死亡的主要原因.
- 虽然已知一些遗传因素,但HomeoboxC10 (HOXC10) 在ESCC病变发生过程中的确切作用尚不清楚.
研究的目的:
- 研究HOXC10在ESCC的发展和进展中的作用.
- 探索HOXC10作为ESCC的诊断和预后生物标志物的潜力.
主要方法:
- 基因表达分析 (Agilent mRNA,qRT-PCR) 和蛋白质验证 (西部斑,免疫组织化学) 用于评估ESCC中的HOXC10水平.
- 对ESCC细胞和体内动物模型进行了体外研究,以评估HOXC10的功能影响.
- 同免疫沉测定确定了HOXC10和叉头盒A3 (FOXA3) 之间的相互作用.
主要成果:
- 在ESCC组织中,HOXC10mRNA水平显著升高,与患者预后不佳相关.
- "HOXC10 knockdown"抑制了ESCC细胞的增殖,入侵和迁移,并在体内抑制了瘤的生长.
- 在ESCC细胞中,HOXC10对FOXA3进行上调,从而增强ESCC细胞中的MAPK信号通路.
结论:
- 在ESCC瘤发生过程中,HOXC10通过通过MAPK通路对FOXA3进行上调,发挥着关键作用.
- HOXC10是食道状细胞癌的潜在诊断和预后标志物.
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