酒精提取物通过MAPK/ERK信号通路产生镇痛作用
Yan Lan1,2, Yu-Kun Zheng3, Liu-Yi Wu3
1Department of Pharmacy, Huangshi Central Hospital, Affiliated Hospital of Hubei Polytechnic University, Huangshi, Hubei, People's Republic of China.
Drug design, development and therapy
|October 25, 2023
概括
葡萄 (Polygonum cuspidatum) 酒精提取物通过抑制脊髓中的MAPK/ERK信号通路,有效地减少炎症性疼痛. 这种传统中医药在开发新的止痛疗法方面表现有前途.
科学领域:
- 药理学 药理学是指药理学的学科.
- 疼痛管理 疼痛管理
- 传统中国医药 传统中国医药
背景情况:
- 传统中国医学 (TCM) 利用多边形 (PC) 具有缓解疼痛的特性.
- 炎症性疼痛是一个重要的健康问题,需要有效的治疗干预措施.
- 了解PC的止痛作用背后的分子机制对于其临床应用至关重要.
研究的目的:
- 在炎症性疼痛模型上研究Polygonum cuspidatum (PC) 酒精提取物的止痛作用.
- 探索潜在的分子机制,专注于参与疼痛感知的信号通路.
- 为了确定PC的止痛功能的潜在治疗点.
主要方法:
- 网络药理学和分子对接被用来预测PC的活性成分的潜在目标.
- 在三种不同的炎症性疼痛小鼠模型中评估了镇痛效应:乙酸诱导的纹,甲素诱导的脚胀,和烯诱导的耳朵胀.
- 脊髓组织分析 (L4-6) 涉及定量实时PCR (RT-qPCR),西斑 (WB) 和免疫组织化学 (IHC) 来评估信号通路激活.
主要成果:
- 网络药理学确定了MAPK/ERK信号通路作为PC的止痛机制的核心.
- 五个PC的关键活性成分表明了与JNK和p38激酶的有利分子对接.
- 在所有三种模型中,PC酒精提取物显著减轻了疼痛行为和炎症反应,抑制了脊髓组织中JNK,ERK,p38和CREB的mRNA和酸化水平.
结论:
- 葡萄 (Polygonum cuspidatum,简称PC) 的酒精提取物具有强大的抗炎和止痛作用.
- 观察到的效应在机制上与脊髓内的MAPK/ERK信号通路的抑制有关.
- PC酒精提取物是治疗炎症性疼痛的有希望的候选药物.
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