关于内性真菌Diaporthe sp. 的二次代谢物的研究 AC1是由托类型的类似物诱导的

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Shikai Zhang¹, Qing Xu¹, Changbo Ji²

¹Department of Microbiology, College of Life Science, Key Laboratory for Agriculture Microbiology, Shandong Agricultural University, Taian, China.

Frontiers in microbiology

|October 25, 2023

概括

植物内真菌Diaporthe sp. 这种真菌. 当AC1被1-甲基-L-氨酸 (1-MT) 诱导时,它会产生具有强大的抗菌和抗癌活性的二次代谢物. 化合物1显示出显著的抗癌作用和适度的抗真菌特性.

科学领域:

自然产品化学自然产品化学
微生物学微生物学
药理学药理学是指药理学的学科.

背景情况:

内部植物性真菌,如Diaporthe sp. 这种真菌. 来自Artemisia argyi的AC1,是生物活性二次代谢产物的来源.
像5-基三 (5-HTP),1-甲基-L-三 (1-MT) 和胺 (TA) 这样的小分子可以诱导真菌代谢物的产生.

研究的目的:

为了研究Diaporthe sp.的二次代谢产物. AC1是由小分子诱导的.
评估这些代谢物的抗菌和细胞毒性活动.
优化诱导条件以提高生物活性.

主要方法:

发酵Diaporthe sp. 的发酵情况在AC1中使用5-HTP,1-MT或TA.
使用牛津杯法进行抗菌和抗真菌检测.
通过MTT测定对癌细胞的细胞毒性评估.
优化1-MT诱导条件 (时间和度).
使用染色学分离和净化化合物.
微稀释测定抗菌和抗真菌活性.
对化合物细胞毒性的MTT测定.

主要成果:

1-MT诱导产生了具有最佳抗菌和细胞毒性作用的二次代谢物.
最佳的1-MT条件:在0.25 mM的注射之前添加.
分离了9种化合物,其中包括3种新化合物 (1-3) 和6种已知的化合物 (4-9).
化合物1对Verticillium dahlia,Fusarium graminearum,Botrytis cinerea表现出中度活性,对Listeria monocytogenes表现出较弱的活性.
化合物1对五种癌细胞系表现出显著的细胞毒性 (IC50:12.2652.52μM).
化合物2和3的活性可以忽略不计;其他化合物具有可检测的生物活性.

结论:

1-MT是一种有效的诱导剂,用于从Diaporthe sp.产生生物活性二次代谢产物. AC1.AC1.AC1.AC1.AC1.AC1.AC1.AC1.AC2.AC1.AC1.AC2.AC1.AC1.AC2.AC1.AC1.AC2.AC.
化合物1是一种有前途的候选物,具有显著的抗癌特性和适度的抗真菌活性.
对化合物1和其他分离的代谢物进行进一步的研究对于潜在的治疗应用是有必要的.

关键词:

1-甲基-L-三甲是什么?幻灯片上传了他的照片.生物活动是生物活动.它们是内性真菌.二次代谢产物二次代谢产物

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