在pe/ppe基因中的功能性遗传变异有助于Mycobacterium结核病血统的多样性和与人类宿主的潜在相互作用
Paula Josefina Gómez-González1, Anna D Grabowska2, Leopold D Tientcheu3
1Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom.
Frontiers in microbiology
|October 25, 2023
概括
在Mycobacterium tuberculosis pe/ppe基因中的遗传多样性被分析到跨血统的基因. 超过50%的这些基因被保存下来,但特定于血统的变异和重组为结核病的致病性和控制提供了洞察力.
科学领域:
- 基因组学就是基因组学.
- 微生物学 微生物学
- 进化生物学 进化生物学
背景情况:
- pe和ppe基因家族约占Mycobacterium结核病编码潜力的10%.
- 这些基因家族与宿主-病原体相互作用有关,但由于它们的重复性和高GC含量,人们对它们的理解仍然不佳.
- 了解pe/ppe基因的遗传多样性对于开发新的结核病预防和治疗策略至关重要.
研究的目的:
- 为了研究169个pe/ppe基因的遗传多样性和保护模式,跨越不同的Mycobacterium结核病血统.
- 为了识别pe/ppe基因家族内的谱系特异变异和结构重组.
- 评估这些基因家族的遗传学信息性和对这些基因家族的致病性潜在影响.
主要方法:
- 测序分析了169个pe/ppe基因,使用来自7个M. tuberculosis和M. bovisBCG系的73个长读组合.
- 提取和分析个别pe/ppe基因对齐以研究多样性和保护.
- 将pe/ppe基因根据相对于H37Rv参考菌株的蛋白质序列保存而分为组.
主要成果:
- 超过50%的pe/ppe基因被发现是跨谱系保存的,pe_pgrs和ppe_mptr子家族的插入/删除 (indels) 导致结构变异.
- 在pe和pe_pgrs基因之间观察到基因重排,包括重复和融合.
- 确定了特定于血统的单核酸多态 (SNP) 和indels,突出显示了血统间的多样性.
结论:
- 保存的性质和pe/ppe基因的谱系特异性变异表明它们在Mycobacterium tuberculosis的遗传学研究中的有用性.
- 鉴定到的结构变异和重组可能会影响病原性,需要进一步调查.
- 对这些复杂的基因家族的更深入的理解可以提供有关结核病原性的见解,并有助于开发控制工具.
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