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转录因子诱导的聚合生物模拟性寡核酸-b-蛋白质微粒

Chloé Grazon^1,2,3, Elisabeth Garanger², Pierre Lalanne²

¹Univ. Bordeaux, CNRS, Bordeaux INP, ISM, UMR 5255, Talence F-33400, France.

Biomacromolecules

|October 25, 2023

概括

研究人员合成了热反应性多-寡核酸生物结合物. 这些自我组装的细胞可以结合蛋白质,并与补充链混合,保持稳定性和温度敏感性,用于先进的应用.

科学领域:

生物结合化学生物结合化学
聚合物科学聚合物科学
纳米技术纳米技术

背景情况:

聚合物米塞尔,特别是来自自然二块的聚合物米塞尔,具有出色的分子识别,生物相容性和生物降解性.
热敏聚合物能够根据温度变化进行自我组装和拆卸,这对于受控输送系统非常有用.

研究的目的:

开发一种易于合成热敏弹性类似弹性多 (ELP) 和寡核酸 (ON) 双阻断生物结合物 (ON-b-ELP) 的方法.
描述ON-b-ELP微粒的自我组装行为和稳定性.
通过杂交和蛋白质结合来证明这些细胞形成复杂结构的能力.

主要方法:

通过铜催化亚酸环添加合成ON-b-ELP二块生物结合物.
在临界微粒温度 (CMT ~30 °C) 以上的微粒形成和自我组装的表征.
评估与补充的ON链的菌根杂交.
使用生物-斯特雷普塔维丁和转录因子-ON相互作用对蛋白质结合能力的评估.

主要成果:

成功合成了热敏的ON-b-ELP二阻生物结合物.
在CMT上方的ON-b-ELP自组装成稳定的小 (直径约50纳米).
微粒在与补充的ON链混合后保持了尺寸和稳定性.
细菌成功地结合了蛋白质,形成了更大的结构,同时保持了温度敏感性.

结论:

开发了一种简单的方法来制造热反应性多-寡核酸生物结合物.
证明了这些生物结合物的潜力,可以形成可调节的,对温度敏感的纳米结构,用于复杂的分子组装.
突出了ON-b-ELP微粒作为通过杂交和蛋白质结合创建复杂生物分子结构的平台的实用性.

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