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Updated: Jul 12, 2025

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N6 - 甲基氨酸改性circSTX6通过调节HNRNPD/ATF3轴和编码144个氨基酸多来促进肝细胞癌的进展

Jiahua Lu^1,2,3,4, Junnan Ru^1,2,3,4, Yunhao Chen^1,2,3,4,5

¹Division of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Peoples Republic of China.

Clinical and translational medicine

|October 25, 2023

概括

经N6-甲基氨酸 (m6A) 修饰的循环合成素6 (circSTX6) 通过HNRNPD/ATF3通路驱动肝细胞癌 (HCC) 恶性. 它的编码蛋白,circSTX6-144aa,也独立促进HCC的进展,提供潜在的治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

循环RNAs (circRNAs) 与肝细胞癌 (HCC) 的发展有关.
循环合成素6 (circSTX6) 在胰腺癌中充当微RNA海绵,但其在HCC中的作用尚不清楚.
研究circSTX6在HCC中的功能,包括蛋白质支架和翻译,至关重要.

研究的目的:

研究circSTX6在肝细胞癌 (HCC) 中的作用和机制.
探索circSTX6编码蛋白在HCC进展中的功能.
为了确定HCC的潜在诊断和治疗目标.

主要方法:

研究了circSTX6及其在HCC组织中的蛋白质表达.
在circSTX6上使用Me-RIP,RIP和光酶试验验证并量化了N6-甲基氨酸 (m6A) 修饰.
使用体外和体外模型评估circSTX6及其蛋白质在HCC中的生物功能,并研究了涉及HNRNPD和ATF3mRNA的分子机制.

主要成果:

CircSTX6及其编码的144氨基酸多 (circSTX6-144aa) 在HCC中表达高,与生存率差相关.
METTL14以一种m6A依赖的方式调节circSTX6表达.
CircSTX6通过海绵化HNRNPD促进了HCC的扩散,瘤性和转移,导致ATF3mRNA衰变. CircSTX6-144aa独立增强了HCC的进展.

结论:

m6A修饰的circSTX6通过HNRNPD/ATF3轴驱动HCC恶性病变.
CircSTX6-144aa独立地促进了HCC的进展.
CircSTX6及其编码的蛋白质是HCC的有希望的生物标志物和治疗点.

关键词:

N6 - 甲基氨酸修改的修改这是一种RNA结合蛋白质.环RNA 环RNA 是一个环RNA.肝细胞癌是肝细胞癌.在mRNA衰变过程中.编码蛋白质的蛋白质编码.

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