相关实验视频

Updated: Jul 12, 2025

Construction of CRISPR Plasmids and Detection of Knockout Efficiency in Mammalian Cells through a Dual Luciferase Reporter System
05:51

Construction of CRISPR Plasmids and Detection of Knockout Efficiency in Mammalian Cells through a Dual Luciferase Reporter System

Published on: December 5, 2020

6.3K

[基于CRISPR/Cas9系统的羊VASA基因敲进载体的构建和验证]

Hua Yang1, Zifei Liu1, Wenli Lü1

  • 1Institute of Sheep and Goat Science, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, Jiangsu, China.

Sheng wu gong cheng xue bao = Chinese journal of biotechnology
|October 25, 2023
PubMed
概括

在绵羊丸发育过程中,VASA基因表达显著增加,在精子生成中发挥关键作用. 研究人员成功地使用CRISPR/Cas9技术创建了VASA基因敲进载体,用于体外研究.

关键词:
这就是CRISPR/Cas9的作用.在CRISPR/dCas9中使用.在VASASA VASA中使用.敲进来,打开一个门.羊群羊群羊群的羊群是什么意思

更多相关视频

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice09:00

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Published on: August 2, 2018

8.2K
CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts06:52

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Published on: September 13, 2022

3.5K

相关概念视频

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
23