单细胞剖析与谱系分析相结合,揭示了阴道和阴道神经对发育中的肠道神经系统的贡献
Jessica Jacobs-Li1, Weiyi Tang1, Can Li1
1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, United States.
eLife
|October 25, 2023
概括
这项研究表明,阴道和阴道神经细胞都对肠道神经系统 (ENS) 有贡献. 令人惊的是,尾后的阴道细胞与尾前的阴道细胞相比,更像是阴道细胞,这表明环境对ENS发育的影响.
科学领域:
- 发育生物学是发展生物学.
- 神经科学是一个神经科学.
- 遗传学 遗传学 是一个
背景情况:
- 肠道神经系统 (ENS) 对胃肠道功能至关重要.
- 肠神经系统主要来自神经细胞,第二次贡献来自神经细胞到后肠.
- 了解这些神经群体的独特贡献和差异化对于ENS发展研究至关重要.
研究的目的:
- 为了比较阴道和神经神经细胞对正在发育的鸟类肠道神经系统和相关的外围质细胞的细胞和分子贡献.
- 调查前和后的神经贡献之间的相似之处和差异.
- 探索发育时间和微环境对ENS中神经细胞分化的影响.
主要方法:
- 在鸟类胚胎中,结合单细胞转录学与轴向水平特定的血统追踪.
- 在胚胎第10天分析了阴道和阴道神经峰衍生的细胞的细胞类型组成和基因表达特征.
- 利用RNA轨迹分析推断发育途径.
主要成果:
- 阴道和阴道神经细胞在胚胎第10天在ENS中产生重叠的神经和质细胞子集.
- 尾后的阴道神经细胞表现出与神经细胞相比,与尾前的阴道神经细胞更相似的转录形状.
- 阴道神经保持了神经/质前池,而神经则显示了胚胎第10天的耗尽的前和丰富的肠质.
结论:
- 交叉神经有助于后肠ENS和相关的外围.
- 脑神经系统中神经源细胞的分化受当地环境和发育时间的影响.
- 这项研究突出了ENS形成期间神经细胞的可塑性和环境响应性.
更多相关视频
06:36Author Spotlight: Isolating and Analyzing Intestinal Cells of Zebrafish Larvae for Investigating Transcriptomic Aspects of Gastrointestinal Development
Published on: November 10, 2023
1.8K
05:11Author Spotlight: Robust Culture of Human Enteric Neurons from Human Pluripotent Stem Cells
Published on: May 17, 2024
1.3K
相关概念视频
01:56Gastrulation
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata will form...
01:30Neurulation
Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the anterior...
01:23Renewal of Intestinal Stem Cells
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...