基于全基因组测序识别由动物血统Mycobacterium orygis引起的人类结核病
Md Rashedul Islam1, Meenu K Sharma1,2, Rupinder KhunKhun3
1National Reference Centre for Mycobacteriology, National Microbiology Laboratory, Public Health Agency of Canada , Winnipeg, Manitoba, Canada.
整个基因组测序确定了Mycobacterium orygis,这是一个导致动物性结核病的新兴病原体. 这种方法可以准确地将M. orygis与其他Mycobacterium结核病复杂物种区分开来,有助于全球卫生监测.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 传染性疾病 传染性疾病
背景情况:
- 结核菌菌复合体 (MTBC) 包括新兴的病原体,如Mycobacterium orygis,它们会在动物和人类中引起动物性结核病.
- 准确识别M. orygis对于了解其对全球健康的影响至关重要,但目前的方法有限.
- 在全球范围内报告了M. orygis,但由于鉴定挑战,其对动物性结核病负担的贡献仍然不清楚.
研究的目的:
- 开发和验证基于全基因组测序 (WGS) 的单核酸多态性 (SNP) 分析,以准确识别M. orygis.
- 使用WGS数据区分M. orygis与其他MTBC物种.
- 为了识别用于M. orygis识别的新型遗传标记.
主要方法:
- 全基因组测序 (WGS) 对来自加拿大的61个M. orygis分离物进行了测序.
- 公开可用的M. orygis序列 (n=56) 被纳入综合分析.
- 进行了单核酸多态 (SNP) 分析,重点关注特定的基因 (gyrB,PPE55,Rv2042c,leuS,mmpL6,mmpS6) 以确定独特的标记物.
主要成果:
- 基于WGS的SNP分析准确地确定了M. orygis并将其与其他MTBC物种区分开来.
- 五种新型SNP (gyrB^277, gyrB^1478, leuS^1064, mmpL6^486, mmpS6^334) 被确定为M. orygis. 的有效遗传标记物.
- 遗传学分析揭示了研究的M. orygis基因组中的遗传多样性,与来自不同地区的人类和动物分离物聚集在一起.
结论:
- 基于WGS的SNP分析为MTBC中识别M. orygis提供了一种高可靠性的方法.
- 已识别的SNP作为有价值的遗传标记,用于准确的M. orygis检测.
- 这种方法将有助于参考和诊断实验室跟踪和管理M. orygis感染,有助于对动物性结核病的理解.
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