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相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Mass Analyzers: Common Types01:19

Mass Analyzers: Common Types

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The quadrupole mass analyzer consists of four cylindrical metal rods arranged in a diamond carrying a DC voltage and a radio-frequency AC voltage. The motion of ions through the quadrupole depends on the field strength, causing only ions of a certain m/z to resonate successfully and strike the detector at a given field strength. Though the transmission rate for these analyzers is high, the exact elemental composition of the sample is not determined because of low resolution; however, they are...
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Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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相关实验视频

Updated: Jul 12, 2025

Large-scale Top-down Proteomics Using Capillary Zone Electrophoresis Tandem Mass Spectrometry
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通过2D MS进行自上而下的蛋白形分析,采用四极检测.

Marek Polák1,2, Michael Palasser3, Alan Kádek1

  • 1Institute of Microbiology of the Czech Academy of Sciences, Prague 14220, Czech Republic.

Analytical chemistry
|October 25, 2023
PubMed
概括

在协调的ICR单元中进行四极检测的二维质谱 (2D MS) 可以在没有离子隔离的情况下进行全面的碎片分析. 这一进步允许进行详细的生物分子研究和无标签量化.

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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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科学领域:

  • 分析化学 分析化学
  • 频谱测量是一种光谱测量.
  • 生物化学 生物化学

背景情况:

  • 二维质谱 (2D MS) 是分析复杂样品的强大技术.
  • 传统的2DMS在FT-ICRMS上依赖于离子调制来关联前体和碎片离子.
  • 通常需要离子隔离,限制多重复合能力.

研究的目的:

  • 在动态协调的ICR单元中引入2DMS的四极检测.
  • 探索四极探测在增强二维MS能力方面的优势.
  • 使用这种新方法开发和应用生物分子分析和量化新的工作流程.

主要方法:

  • 在一个协调的ICR单元中实现具有四极检测的2DMS.
  • 调整数据处理技术,以准确地将频率转换为质量.
  • 使用ECD碎片化对共价标记的ubiquitin进行自上而下分析的应用.

主要成果:

  • 在一个协调的ICR细胞中成功展示了具有四极检测的2DMS.
  • 四极检测的确立优势,用于全面的碎片化分析.
  • 开发了一种工作流程,用于对生物分子异型的无标签相对量化.

结论:

  • 四极检测通过消除离子隔离的需要,显著提高了二维MS的能力.
  • 这种方法为详细的生物分子表征提供了一个强大的平台.
  • 开发的工作流促进了对生物分子异型的无标签量化,推动了蛋白质学研究.