HuR诱导的circ_0082319通过通过miR-505-3pp提升PTK2促进肝细胞癌
Chuntang Qin1, Shuyan Liu2, Weibin Chen3
1Department of Interventional, The First Affiliated Hospital of Henan Polytechnic University (Jiaozuo Second People's Hospital), Democratic South Road 17, Jiefang District, Jiaozuo, 454000, China. jzyueliangchuan@126.com.
Naunyn-Schmiedeberg's archives of pharmacology
|October 25, 2023
概括
循环RNAcirc_0082319通过海绵化miR-505-3p促进肝细胞癌 (HCC) 来上调PTK2. HuR增强了circ_0082319,推动了HCC的进展,并暗示了一个治疗点.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 肝细胞癌 (HCC) 仍然是一个重大的全球健康挑战.
- 了解推动HCC进展的分子机制对于开发有效疗法至关重要.
研究的目的:
- 研究circ_0082319在肝细胞癌 (HCC) 进展中的生物学作用和机制.
- 阐明涉及circ_0082319的调节网络,包括微RNA-505-3p (miR-505-3p) 和蛋白氨酸激酶2 (PTK2).
主要方法:
- 定量实时PCR和西布洛特用于评估circ_0082319,miR-505-3p,PTK2和HuR的表达水平.
- 在体外测试 (MTT,EDU,流细胞计,Transwell,管形成) 以评估细胞活力,增殖,细胞亡,侵袭和血管生成.
- 双 luciferase 记者和RNA 免疫沉降测定以确认分子相互作用.
- 在体内异种移植瘤模型,以评估对瘤生长的影响.
主要成果:
- 在HCC组织和细胞系中,Circ_0082319,PTK2和HuR被上调,而miR-505-3p被下调.
- Knockdown of circ_0082319 抑制了 HCC 细胞的增殖,侵入,血管生成,并促进了细胞亡.
- Circ_0082319作为miR-505-3p的分子海绵,从而调节PTK2的表达.
- 在HCC细胞中HuR正调节的circ_0082319表达.
- 由HuR介导的circ_0082319/miR-505-3p/PTK2轴促进了HCC的进展.
结论:
- Circ_0082319 在HCC进展中起着关键的致癌作用.
- 这种HuR/circ_0082319/miR-505-3p/PTK2通路代表了HCC治疗的潜在治疗标.
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