在反复的植入失败中构建枢纽转录因子-microRNAs-messenger RNA调节网络
Jiahuan Luo1,2,3,4, Rongxia Huang5, Pengying Xiao6
1Department of Reproductive Genetics, The First Affiliated Hospital of Kunming Medical University, No. 295, Xichang Road, Wuhua District, Kunming, China.
Journal of assisted reproduction and genetics
|October 25, 2023
概括
复发性植入失败 (RIF) 是体外受精 (IVF) 的一个挑战. 这项研究确定了三个关键的转录因子 (TF) EOMES,NKX2-1和POU5F1 作为RIF诊断和治疗的潜在生物标志物.
科学领域:
- 生殖生物学和遗传学 生殖生物学和遗传学
- 生物信息学和计算生物学
背景情况:
- 重复植入失败 (RIF) 影响全球高达10%的体外受精 (IVF) 患者.
- RIF的潜在分子机制在很大程度上是未知的.
- 确定关键的监管因素对于理解和处理RIF至关重要.
研究的目的:
- 使用生物信息学识别与反复植入失败 (RIF) 相关的枢纽转录因子 (TF).
- 阐明RIF所涉及的分子通路和调节网络.
- 发现RIF的潜在诊断生物标志物和治疗点.
主要方法:
- 从基因表达综合 (GEO) 数据库下载并分析人类子宫内膜组织数据集 (mRNA和miRNA).
- 确定了与RIF相关的差异表达转录因子 (DETF),miRNA和基因.
- 对DETF进行了功能丰富分析,并构建了蛋白质-蛋白质相互作用网络.
- 通过网络分析确定了枢纽TF,并使用Cytoscape构建了一个TF-miRNA-mRNA监管网络.
主要成果:
- 确定了57个差异表达的转录因子 (DETF),主要参与转录调节.
- 丰富分析显示,DETFs与癌症和信号通路中的转录错误调节等途径有关.
- EOMES,NKX2-1和POU5F1被确定为关键的枢纽转录因子.
- 构建了一个涉及三个枢纽TF,四个miRNA和四个基因的调控网络.
结论:
- 确定了EOMES,NKX2-1和POU5F1作为RIF诊断的潜在分子生物标志物.
- 这些枢纽TF可以作为复发性植入失败的未来治疗点.
- 这项研究为RIF的分子机制提供了新的见解,需要进一步的实验验证.
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